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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum
Authors: Sharifuzzaman, S M
Abbass, Amany A
Tinsley, John W
Austin, Brian
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Keywords: Probiotic
fish pathogens
Issue Date: Jan-2011
Publisher: Elsevier
Citation: Sharifuzzaman SM, Abbass AA, Tinsley JW & Austin B (2011) Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum, Fish and Shellfish Immunology, 30 (1), pp. 347-353.
Abstract: The efficacy of cellular components of probiotics Kocuria SM1 and Rhodococcus SM2 to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against vibriosis was assessed. Groups of fish (average weight = 10–15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e. 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ECPs), cell wall proteins (CWPs) and whole cell proteins (WCPs) of SM1 and SM2, respectively, or with 0.1 ml of phosphate-buffered saline (PBS) to serve as the control. Seven days after administration, fish from each group were challenged i.p. with 0.1 ml of a suspension in PBS of 3 × 105 cells ml−1 per fish of Vibrio anguillarum. Use of CWPs and WCPs demonstrated significantly (P < 0.05) better protection against V. anguillarum insofar as mortalities were reduced to 11–17% [relative percent survival (RPS) = 80–87%], although ECPs fared less well (mortalities = 33–38%; RPS = 56–62%; P > 0.05), compared to 86% mortalities of the controls. The mode of action reflected activation of innate immune factors by CWPs and WCPs, demonstrating significantly (P < 0.05) increased expression of respiratory burst (optical density; OD550nm) from 0.039 to 0.043–0.045, peroxidase (OD550nm) from 0.26 to 0.37–0.55, and bacterial killing activities (i.e. percentage of surviving bacteria reduced from 79% to 56–57% for SM2). Moreover, an elevation of leucocyte number (from 1.93% to 1.98–2.93%; P > 0.05) and immunoglubolin level (from 27 mg ml−1 to 28.5–33 mg ml−1; P > 0.05) were observed with the experimental groups. These results indicate that cell components of the probiotics stimulate an immune response.
Type: Journal Article
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Rights: Published in Fish and Shellfish Immunology by Elsevier. Fish and Shellfish Immunology, Volume 30, Issue 1, January 2011, pp. 347 - 353.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Fish and Shellfish Immunology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fish and Shellfish Immunology, VOL 30, ISSUE 1, (January 2011). DOI: 10.1016/j.fsi.2010.11.005.
Affiliation: Heriot-Watt University
Benha University
Heriot-Watt University

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