Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3044
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dc.contributor.authorMinghetti, Matteoen_UK
dc.contributor.authorLeaver, Michaelen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2012-09-03T23:27:33Z-
dc.date.available2012-09-03T23:27:33Z-
dc.date.issued2011-03en_UK
dc.identifier.urihttp://hdl.handle.net/1893/3044-
dc.description.abstractThe regulatory control mechanisms of lipid and fatty acid metabolism were investigated in Atlantic salmon. We identified sterol regulatory element binding protein (SREBP) genes in salmon and characterised their response, and the response of potential target and other regulatory genes including liver X receptor (LXR), to cholesterol and long-chain polyunsaturated fatty acids (LC-PUFA) in the salmon established cell line, SHK-1. Two cDNAs for SREBPs homologous to mammalian SREBP-1 and SREBP-2 were characterised. We identified three groups of genes whose expression responded differently to the treatments. One group of genes, including cholesterol biosynthetic genes, showed increased expression in response to lipid depletion but supplementary cholesterol or LC-PUFA had no further effect. The expression of a second group of genes belonging to fatty acid biosynthetic pathways, included fatty acid synthase, Δ6 and Δ5 fatty acyl desaturases, also increased after lipid depletion but this was negated by cholesterol or by LC-PUFA supplementation. The expression of a third group of genes including acyl-CoA oxidase, HMG-CoA reductase and Elovl5 elongase was increased by cholesterol treatment but was not affected by lipid depletion or by LC-PUFA. This same pattern of expression was also shown by liver X receptor (LXR), indicating that acyl-CoA oxidase, HMG-CoA reductase and Elovl5 are possible direct targets of LXR. This suggests that salmon Elovl5 may be regulated differently from mammalian Elovl5, which is an indirect target of LXR, responding to LXR-dependent increases in SREBP-1.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMinghetti M, Leaver M & Tocher DR (2011) Transcriptional control mechanisms of genes of lipid and fatty acid metabolism in the Atlantic salmon (Salmo salar L.) established cell line, SHK-1. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1811 (3), pp. 194-202. https://doi.org/10.1016/j.bbalip.2010.12.008en_UK
dc.rightsPublished in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1811, Issue 3, March 2011, pp. 194 - 202.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1811, ISSUE 3, (March 2011). DOI: 10.1016/j.bbalip.2010.12.008en_UK
dc.subjectAtlantic salmonen_UK
dc.subjectSHK-1 cell lineen_UK
dc.subjectTranscription factorsen_UK
dc.subjectSREBPen_UK
dc.subjectLXRen_UK
dc.subjectPPARen_UK
dc.subjectNuclear receptorsen_UK
dc.subjectgene expressionen_UK
dc.subjectlipid metabolismen_UK
dc.subjectfatty acidsen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutritionen_UK
dc.titleTranscriptional control mechanisms of genes of lipid and fatty acid metabolism in the Atlantic salmon (Salmo salar L.) established cell line, SHK-1en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.bbalip.2010.12.008en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1811en_UK
dc.citation.issue3en_UK
dc.citation.spage194en_UK
dc.citation.epage202en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date28/12/2010en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000289270200010en_UK
dc.identifier.scopusid2-s2.0-78751648416en_UK
dc.identifier.wtid837231en_UK
dc.contributor.orcid0000-0002-3155-0844en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted1990-01-01en_UK
dcterms.dateAccepted1990-01-01en_UK
dc.date.filedepositdate2011-06-03en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMinghetti, Matteo|en_UK
local.rioxx.authorLeaver, Michael|0000-0002-3155-0844en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-06-03en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-06-03|en_UK
local.rioxx.filenameMinghetti et al(FINAL).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1388-1981en_UK
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