Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3042
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dc.contributor.authorMorais, Sofiaen_UK
dc.contributor.authorMourente, Gabrielen_UK
dc.contributor.authorOrtega, Aurelioen_UK
dc.contributor.authorTocher, Jamie Aen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2013-06-09T04:35:50Z-
dc.date.available2013-06-09T04:35:50Z-
dc.date.issued2011-03-15en_UK
dc.identifier.urihttp://hdl.handle.net/1893/3042-
dc.description.abstractThe concentration of n-3 long-chain polyunsaturated fatty acids (LC-PUFA) in neural tissues is known to be crucial for effective prey capture from the time of first feeding in marine fish larvae. Furthermore, tissues of tunids, including Atlantic bluefin tuna, have relatively high levels of DHA (docosahexaenic acid, 22:6n-3) and a high ratio of DHA:EPA (eicosapentaenoic acid; 20:5n-3) compared to most other species. Although the lipid biochemistry underpinning the high DHA:EPA ratio in tuna is unclear, it has been generally assumed that they must selectively accumulate and retain DHA in their tissues. In the present study, we investigated lipid and fatty acid metabolism during early development of Atlantic bluefin tuna and determined the changes in lipid content, lipid class composition and total, phospholipid and neutral lipid fatty acid profiles in unfed larvae during yolk sac utilisation. In addition, we studied the LC-PUFA biosynthesis pathway by quantifying expression of fatty acyl desaturase and elongase genes. To this end, we cloned and functionally characterized two cDNAs by heterologous expression in yeast, showing them to code for a Δ6 desaturase and Elovl5 elongase, respectively, that could both be involved in the conversion of EPA to DHA. The level of DHA was maintained, but the proportion of EPA declined, and so the DHA:EPA ratio increased in bluefin tuna larvae during yolk sac utilization. Although this could be the result of relative retention of DHA during a period of generally high fatty acid oxidation and utilization, there was also a great increase in desaturase and elongase expression with larval development. This suggests that increased activity of these enzymes is important for the normal development of tuna larvae related to the provision of adequate DHA for the formation of biomembranes, particularly in neural (brain and eye) tissues.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMorais S, Mourente G, Ortega A, Tocher JA & Tocher DR (2011) Expression of fatty acyl desaturase and elongase genes, and evolution of DHA:EPA ratio during development of unfed larvae of Atlantic bluefin tuna (Thunnus thynnus L.). Aquaculture, 313 (1-4), pp. 129-139. http://www.sciencedirect.com/science/journal/00448486; https://doi.org/10.1016/j.aquaculture.2011.01.031en_UK
dc.rightsPublished in Aquaculture by Elsevier. Aquaculture, Volume 313, Issues 1-4, March 2011, pp. 129 - 139.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, VOL 313, ISSUE 1-4, (March 2011). DOI: 10.1016/j.aquaculture.2011.01.031en_UK
dc.subjectAtlantic bluefin tunaen_UK
dc.subjectyolk sac larvaeen_UK
dc.subjectlipid content and compositionen_UK
dc.subjectfatty acid compositionen_UK
dc.subjectfatty acyl desaturaseen_UK
dc.subjectfatty acyl elongaseen_UK
dc.subjectgene expressionen_UK
dc.subjectdevelopmenten_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutritionen_UK
dc.titleExpression of fatty acyl desaturase and elongase genes, and evolution of DHA:EPA ratio during development of unfed larvae of Atlantic bluefin tuna (Thunnus thynnus L.)en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.aquaculture.2011.01.031en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume313en_UK
dc.citation.issue1-4en_UK
dc.citation.spage129en_UK
dc.citation.epage139en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/journal/00448486en_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date28/01/2011en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Cadizen_UK
dc.contributor.affiliationSpanish Institute of Oceanography (IEO)en_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000289018500018en_UK
dc.identifier.scopusid2-s2.0-79952068915en_UK
dc.identifier.wtid837142en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted1990-01-01en_UK
dcterms.dateAccepted1990-01-01en_UK
dc.date.filedepositdate2011-06-03en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMorais, Sofia|en_UK
local.rioxx.authorMourente, Gabriel|en_UK
local.rioxx.authorOrtega, Aurelio|en_UK
local.rioxx.authorTocher, Jamie A|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-06-03en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-06-03|en_UK
local.rioxx.filenameMorais et al (FINAL).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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