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http://hdl.handle.net/1893/30102| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Species-Specific Marker Discovery in Tilapia |
| Author(s): | Syaifudin, Mochamad Bekaert, Michaël Taggart, John B Bartie, Kerry L Wehner, Stefanie Palaiokostas, Christos Khan, M G Q Selly, Sarah-Louise C Hulata, Gideon D’Cotta, Helena Baroiller, Jean-Francois McAndrew, Brendan J Penman, David J |
| Issue Date: | 2019 |
| Date Deposited: | 18-Sep-2019 |
| Citation: | Syaifudin M, Bekaert M, Taggart JB, Bartie KL, Wehner S, Palaiokostas C, Khan MGQ, Selly SC, Hulata G, D’Cotta H, Baroiller J, McAndrew BJ & Penman DJ (2019) Species-Specific Marker Discovery in Tilapia. Scientific Reports, 9, Art. No.: 13001. https://doi.org/10.1038/s41598-019-48339-2 |
| Abstract: | Tilapias (family Cichlidae) are of importance in aquaculture and fisheries. Hybridisation and introgression are common within tilapia genera but are difficult to analyse due to limited numbers of species-specific genetic markers. We tested the potential of double digested restriction-site associated DNA (ddRAD) sequencing for discovering single nucleotide polymorphism (SNP) markers to distinguish between 10 tilapia species. Analysis of ddRAD data revealed 1,371 shared SNPs in the de novo-based analysis and 1,204 SNPs in the reference-based analysis. Phylogenetic trees based on these two analyses were very similar. A total of 57 species-specific SNP markers were found among the samples analysed of the 10 tilapia species. Another set of 62 species-specific SNP markers was identified from a subset of four species which have often been involved in hybridisation in aquaculture: 13 for Oreochromis niloticus, 23 for O. aureus, 12 for O. mossambicus and 14 for O. u. hornorum. A panel of 24 SNPs was selected to distinguish among these four species and validated using 91 individuals. Larger numbers of SNP markers were found that could distinguish between the pairs of species within this subset. This technique offers potential for the investigation of hybridisation and introgression among tilapia species in aquaculture and in wild populations. |
| DOI Link: | 10.1038/s41598-019-48339-2 |
| Rights: | his article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Licence URL(s): | http://creativecommons.org/licenses/by/4.0/ |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| s41598-019-48339-2.pdf | Fulltext - Published Version | 3.57 MB | Adobe PDF | View/Open |
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