Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2975
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells
Authors: Clark, Karl J
Carlson, Daniel F
Leaver, Michael
Foster, Linda K
Fahrenkrug, Scott C
Contact Email: mjl1@stir.ac.uk
Issue Date: Mar-2009
Publisher: Oxford University Press
Citation: Clark KJ, Carlson DF, Leaver M, Foster LK & Fahrenkrug SC (2009) Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells, Nucleic Acids Research, 37 (4), pp. 1239-1247.
Abstract: The Tc1/mariner family of DNA transposons is widespread across fungal, plant and animal kingdoms, and thought to contribute to the evolution of their host genomes. To date, an active Tc1 transposon has not been identified within the native genome of a vertebrate. We demonstrate that Passport, a native transposon isolated from a fish (Pleuronectes platessa), is active in a variety of vertebrate cells. In transposition assays, we found that the Passport transposon system improved stable cellular transgenesis by 40-fold, has an apparent preference for insertion into genes, and is subject to overproduction inhibition like other Tc1 elements. Passport represents the first vertebrate Tc1 element described as both natively intact and functionally active, and given its restricted phylogenetic distribution, may be contemporaneously active. The Passport transposon system thus complements the available genetic tools for the manipulation of vertebrate genomes, and may provide a unique system for studying the infiltration of vertebrate genomes by Tc1 elements
Type: Journal Article
URI: http://hdl.handle.net/1893/2975
DOI Link: http://dx.doi.org/10.1093/nar/gkn1025
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Nucleic Acids Research following peer review. The definitive publisher-authenticated version Nucleic Acids Research (2009) 37 (4), 1239-1247 is available online at: http://nar.oxfordjournals.org/content/37/4/1239
Affiliation: University of Minnesota
University of Minnesota
Aquaculture
University of Minnesota
University of Minnesota

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