Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2933
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A vertebrate fatty acid desaturase with delta5 and delta6 activities
Authors: Hastings, Nicola
Agaba, Morris K
Tocher, Douglas R
Leaver, Michael
Dick, James R
Sargent, John R
Teale, Alan J
Contact Email: drt1@stir.ac.uk
Keywords: Zebrafish
Danio rerio
Fatty acyl desaturase
Bifunctional delta-6/delta-5
Polyunsaturated fatty acids
Biosynthesis
cDNA cloning
Functional characterisation
Issue Date: Dec-2001
Publisher: National Academy of Sciences (USA)
Citation: Hastings N, Agaba MK, Tocher DR, Leaver M, Dick JR, Sargent JR & Teale AJ (2001) A vertebrate fatty acid desaturase with delta5 and delta6 activities, Proceedings of the National Academy of Sciences, 98 (25), pp. 14304-14309.
Abstract: Δ5 and Δ6 fatty acid desaturases are critical enzymes in the pathways for the biosynthesis of the polyunsaturated fatty acids (PUFA) arachidonic, eicosapentanoic and docosahexanoic acids. They are encoded by distinct genes in mammals and in Caenorhabditis elegans. This paper describes a cDNA isolated from zebrafish (Danio rerio) with high similarity to mammalian Δ6 desaturase genes. The 1590 bp sequence specifies a protein that, in common with other fatty acid desaturases, contains an N-terminal cytochrome b5 domain and three histidine boxes, believed to be involved in catalysis. When the zebrafish cDNA was expressed in Saccharomyces cerevisae it conferred the ability to convert linoleic acid (18:2n-6) and α-linolenic acid (18:3n-3) to their corresponding Δ6 desaturated products, 18:3n-6 and 18:4n-3. However, in addition, it conferred on the yeast the ability to convert di-homo-γ-linoleic acid (20:3n-6) and eicosatetraenoic acid (20:4n-3) to arachidonic acid (20:4n-6) and eicosapentanoic acid (20:5n-3), respectively, indicating that the zebrafish gene encodes an enzyme having both Δ5 and Δ6 desaturase activity. This is the first report of a functionally characterized desaturase enzyme of fish, and the first report of a fatty acid desaturase in any species with both Δ5 and Δ6 activity. The zebrafish Δ5/Δ6 desaturase may represent a component of a prototypic vertebrate PUFA biosynthesis pathway.
Type: Journal Article
URI: http://hdl.handle.net/1893/2933
URL: http://www.pnas.org/content/98/25/14304.abstract
DOI Link: http://dx.doi.org/10.1073/pnas.251516598
Rights: Published in Proceedings of the National Academy of Sciences of the United States of America (PNAS). Copyright: © 2001 by the National Academy of Sciences.
Affiliation: University of Stirling
University of Stirling
Aquaculture
Aquaculture
Aquaculture
University of Stirling
University of Stirling

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