Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2931

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Effects of dietary vegetable oil on atlantic salmon hepatocyte fatty acid desaturation and liver fatty acid compositions
Authors: Tocher, Douglas R
Bell, J Gordon
Dick, James R
Crampton, Viv
Contact Email: drt1@stir.ac.uk
Keywords: Atlantic salmon
Salmo salar
Fish oil
Vegetable oil
Linseed oil
Rapeseed oil
Mixture design
Liver
Hepatocytes
Lipid metabolism
Polyunsaturated fatty acid
biosynthesis
Desaturation
Elongation
Issue Date: Jul-2003
Publisher: Springer / American Oil Chemists' Society (AOCS)
Citation: Tocher DR, Bell JG, Dick JR & Crampton V (2003) Effects of dietary vegetable oil on atlantic salmon hepatocyte fatty acid desaturation and liver fatty acid compositions, Lipids, 38 (7), pp. 723-732.
Abstract: Fatty acyl desaturase activities, involved in the conversion of the C18 EFA, 18:2n-6 and 18:3n-3, to the highly unsaturated fatty acids (HUFA) 20:4n-6, 20:5n-3 and 22:6n-3, are known to be under nutritional regulation. Specifically, the activity of the desaturation/elongation pathway is depressed when animals, including fish, are fed fish oils rich in n-3HUFA compared to animals fed vegetable oils rich in C18 EFA. The primary aims of the present study were a) to establish the relative importance of product inhibition (n-3HUFA) versus increased substrate concentration (C18 EFA) and, b) to determine whether 18:2n-6 and 18:3n-3 differ in their effects, on the hepatic fatty acyl desaturation/elongation pathway in Atlantic salmon (Salmo salar). Smolts were fed ten experimental diets containing blends of two vegetable oils, linseed (LO) and rapeseed oil (RO), and fish oil (FO) in a triangular mixture design for 50 weeks. Fish were sampled after 32 and 50 weeks, lipid and fatty acid composition of liver determined, fatty acyl desaturation/elongation activity estimated in hepatocytes using [1-14C]18:3n-3 as substrate, and the data subjected to regression analyses. Dietary 18:2n-6 was positively correlated, and n-3HUFA negatively correlated, with lipid content of liver. Dietary 20:5n-3 and 22:6n-3 were positively correlated with liver fatty acids with a slope greater than unity suggesting relative retention and deposition of these HUFA. In contrast, dietary 18:2n-6 and 18:3n-3 were positively correlated with liver fatty acids with a slope of less than unity suggesting metabolism via β-oxidation and/or desaturation/elongation. Consistent with this, fatty acyl desaturation/elongation in hepatocytes was significantly increased by feeding diets containing vegetable oils. Dietary 20:5n-3 and 22:6n-3 levels were negatively correlated with hepatocyte fatty acyl desaturation. At 32 weeks, 18:2n-6 but not 18:3n-3, was positively correlated with hepatocyte fatty acyl desaturation activity whereas the reverse was true at 50 weeks. The data indicate that both feedback inhibition through increased n-3HUFA and decreased C18 fatty acyl substrate concentration are probably important in determining hepatocyte fatty acyl desaturation activities, and that 18:2n-6 and 18:3n-3 may differ in their effects on this pathway.
Type: Journal Article
URI: http://hdl.handle.net/1893/2931
URL: http://www.springerlink.com/content/0024-4201/
DOI Link: http://dx.doi.org/10.1007/s11745-003-1120-y
Rights: Published in Lipids by Springer / American Oil Chemists' Society (AOCS).; The final publication is available at www.springerlink.com
Affiliation: Aquaculture
Aquaculture
Aquaculture
EWOS Innovation

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