Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2929

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Effects of dichlorvos and formalin on fatty acid metabolism of rainbow trout (Oncorhynchus mykiss) skin cells in primary culture
Authors: Ghioni, Cristina
Tocher, Douglas R
Sargent, John R
Contact Email: drt1@stir.ac.uk
Keywords: Rainbow trout
Oncorhynchus mykiss
Skin
Primary cell culture
Lipid composition
Fatty acid composition
Metabolism
Dichlorvos
Formalin
Issue Date: May-1998
Publisher: Springer
Citation: Ghioni C, Tocher DR & Sargent JR (1998) Effects of dichlorvos and formalin on fatty acid metabolism of rainbow trout (Oncorhynchus mykiss) skin cells in primary culture, Fish Physiology and Biochemistry, 18 (3), pp. 241-252.
Abstract: The effects of sub-lethal doses of dichlorvos and formalin, antimicrobial/ parasitic agents used in aquaculture, on lipid composition and metabolism of rainbow trout skin cells in primary culture were investigated. [1-14C]Stearic (18:0), [1-14C]linoleic (18:2n-6) and [1-14C]linolenic (18:3n-3) acids were used as tracers to determine effects on fatty acid incorporation and metabolism. Formalin increased cell numbers and reduced the lipid content of the cells and the incorporation of radioactive fatty acids. The effects of dichlorvos were qualitatively similar but quantitatively less. Formalin induced relatively small but significant changes in lipid class composition including a decreased proportion of phosphatidycholine with increased proportions of phosphatidylethanolamine and phosphatidylserine. Dichlorvos had no significant effect on lipid class compositions. The trout primary skin cells expressed substantial Δ9, Δ6 and Δ5 fatty acyl desaturase activities. Although, as expected, the cells were more active towards [1-14C]18:3n-3, the cells were unusually active towards [1-14C]18:2n-6. Both dichlorvos and, especially, formalin appeared to significantly inhibit Δ9 and Δ6 desaturase. Changes in the distribution of radioactivity between individual phospholipid classes was also influenced by formalin and dichlorvos, and this may be related to changes in desaturase activity. This study has shown that topically active agents used in aquaculture, formalin and dichlorvos, had a range of effects on the rainbow trout skin cell cultures that may affect cell proliferation and lipid and fatty acid metabolism. Both agents significantly inhibited desaturation of fatty acids, particularly of 18:2n-6 to 20:4n-6 and, as 20:4n-6 is a major eicosanoid precursor in fish and considering the importance of eicosanoids in the biochemistry of skin ,it is suggested that these agents may have direct effects on fish skin that may have important consequences for fish health in gen
Type: Journal Article
URI: http://hdl.handle.net/1893/2929
URL: http://www.springerlink.com/content/0920-1742/
DOI Link: http://dx.doi.org/10.1023/A:1007730730655
Rights: Published in Fish Physiology and Biochemistry by Springer.; The final publication is available at www.springerlink.com
Affiliation: University of Stirling
Aquaculture
University of Stirling

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