STORRE
STORRE: Stirling Online Research Repository
Please use this identifier to cite or link to this item:
http://hdl.handle.net/1893/29234| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Recombinase polymerase amplification assay for rapid detection of Monkeypox virus |
| Author(s): | Davi, Saskia Dede Kissenkötter, Jonas Faye, Martin Böhlken-Fascher, Susanne Stahl-Hennig, Christiane Faye, Oumar Faye, Ousmane Sall, Amadou A Weidmann, Manfred Ademowo, Olusegun George Hufert, Frank T Czerny, Claus-Peter Abd El Wahed, Ahmed |
| Contact Email: | m.w.weidmann@stir.ac.uk |
| Keywords: | Recombinase polymerase amplification assay Monkeypox Virus mobile suitcase point of need rapid detection system |
| Issue Date: | Sep-2019 |
| Date Deposited: | 5-Apr-2019 |
| Citation: | Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. https://doi.org/10.1016/j.diagmicrobio.2019.03.015 |
| Abstract: | In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent. RPA was operated at a single constant temperature of 42°C and produced results within 3 to 10 minutes. The MPXV-RPA-assay was highly sensitive with a limit of detection of 16 DNA molecules/μl. The clinical performance of the MPXV-RPA-assay was tested using 47 sera and whole blood samples from humans collected during the recent MPXV outbreak in Nigeria as well as 48 plasma samples from monkeys some of which were experimentally infected with MPXV. The specificity of the MPXV-RPA-assay was 100% (50/50), while the sensitivity was 95% (43/45). This new MPXV-RPA-assay is fast and can be easily utilised at low resource settings using a solar powered mobile suitcase laboratory. |
| DOI Link: | 10.1016/j.diagmicrobio.2019.03.015 |
| Rights: | This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. DOI: https://doi.org/10.1016/j.diagmicrobio.2019.03.015 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http:// creativecommons.org/licenses/by-nc-nd/4.0/ |
| Licence URL(s): | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| MPXV-Manuscript2 after revierwers corrections.pdf | Fulltext - Accepted Version | 941.72 kB | Adobe PDF | View/Open |
This item is protected by original copyright |
A file in this item is licensed under a Creative Commons License
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.