Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2911

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Low C18 to C20 fatty acid elongase activity and limited conversion of stearidonic acid, 18:4(n-3), to eicosapentaenoic acid, 20:5(n-3), in a cell line from the turbot, Scophthalmus maximus
Authors: Ghioni, Cristina
Tocher, Douglas R
Bell, Michael
Dick, James R
Sargent, John R
Contact Email: drt1@stir.ac.uk
Keywords: Fish
Cell culture
Fatty acids
Polyunsaturated fatty acid
biosynthesis
Desaturation
Elongation
Metabolism
AS cell line
TF cell line
Atlantic salmon
Turbot
Issue Date: 25-Feb-1999
Publisher: Elsevier
Citation: Ghioni C, Tocher DR, Bell M, Dick JR & Sargent JR (1999) Low C18 to C20 fatty acid elongase activity and limited conversion of stearidonic acid, 18:4(n-3), to eicosapentaenoic acid, 20:5(n-3), in a cell line from the turbot, Scophthalmus maximus, 1437 (2), pp. 170-181.
Abstract: The TF cell line, derived from a top predatory, carnivorous marine teleost, the turbot (Scophthalmus maximus), is known to have a limited conversion of C18 to C20 polyunsaturated fatty acids (PUFA). To illuminate the underlying processes, we studied the conversions of stearidonic acid, 18:4(n-3), and its elongation product, 20:4(n-3), in TF cells and also in a cell line, AS, derived from Atlantic salmon (Salmo salar), by adding unlabelled (25 uM), U-14C (1 uM) or deuterated (d5; 25 uM) fatty acids. Stearidonic acid, 18:4(n-3), was metabolised to 20:5(n-3) in both cells lines, but more so in AS than in TF cells. Delta-5 desaturation was more active in TF cells than in AS cells, whereas C18 to C20 elongation was much reduced in TF as compared to AS cells. Only small amounts of docosahexaenoic acid (22:6(n-3)) were produced by both cell lines, although there was significant production of 22:5(n-3) in both cultures, especially when 20:4(n-3) was supplemented. We conclude that limited elongation of C18 to C20 fatty acids rather than limited fatty acyl Delta-5 desaturation accounts for the limited rate of conversion of 18:3(n-3) to 20:5(n-3) in the turbot cell line, as compared to the Atlantic salmon cell line. The results can account for the known differences in conversions of C18 to C20 PUFA by the turbot and the Atlantic salmon in vivo.
Type: Journal Article
URI: http://hdl.handle.net/1893/2911
URL: http://www.sciencedirect.com/science/journal/13881981
DOI Link: http://dx.doi.org/10.1016/S1388-1981(99)00010-4
Rights: Published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1437, Issue 2, February 1999, pp. 170 - 181; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1437, ISSUE 2, February 1999. DOI 10.1016/S1388-1981(99)00010-4
Affiliation: University of Stirling
Aquaculture
Aquaculture
Aquaculture
University of Stirling

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