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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Effect of partial substitution of dietary fish oil by vegetable oils on desaturation and beta-oxidation of [1-14C]18:3n-3 (LNA) and [1-14C]20:5n-3 (EPA) in hepatocytes and enterocytes of European sea bass (Dicentrarchus labrax L.)
Authors: Mourente, Gabriel
Dick, James R
Bell, J Gordon
Tocher, Douglas R
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Keywords: European sea bass
Dicentrarchus labrax
fish oil
vegetable oil
lipid metabolism
fatty acid meatabolism
Issue Date: Jul-2005
Publisher: Elsevier
Citation: Mourente G, Dick JR, Bell JG & Tocher DR (2005) Effect of partial substitution of dietary fish oil by vegetable oils on desaturation and beta-oxidation of [1-14C]18:3n-3 (LNA) and [1-14C]20:5n-3 (EPA) in hepatocytes and enterocytes of European sea bass (Dicentrarchus labrax L.), Aquaculture, 248 (41000), pp. 173-186.
Abstract: The increasing worldwide aquaculture output and concomitant decrease in the stocks of feed-grade fish used for fish oil production has made fish oil replacement in feeds a priority for the aquaculture industry. The regulation of fatty acid metabolism in fish is important in order to determine strategies for the best use of plant oils in diets for commercially important cultured fish species. We have studied the desaturation/elongation and β-oxidation of 14C-linolenic (LNA) and 14C-eicosapentaenoic (EPA) acids in hepatocytes and pyloric caecal enterocytes in European sea bass fed diets with partial substitution (60%) of fish oil (FO) with vegetable oils (rapeseed, linseed and palm oil) blended in different proportions, for 64 weeks. The rate of desaturation of 14C‑LNA was very low in hepatocytes from all treatments and no significant differences were observed among treatments. The rate of desaturation of 14C‑LNA in enterocytes was higher than that in hepatocytes but still low (less than 5% of total radioactivity recovered). The desaturation of 14C‑EPA in enterocytes was also higher than in hepatocytes, but again was low and no significant differences were found among treatments. The rates of ß‑oxidation of 14C-LNA and 14C-EPA were much higher than the rates of desaturation in both hepatocytes and enterocytes, however, no significant differences were observed in either hepatocytes or enterocytes among treatments. The rates of β-oxidation of 14C-LNA were considerably higher than those of 14C-EPA in both hepatocytes and enterocytes. In conclusion, European sea bass (a carnivorous marine fish), showed very low desaturation and elongation of LNA to EPA and DHA, and EPA to DHA, higher β−oxidation of LNA than EPA, and all desaturation and oxidation activities were significantly higher in enterocytes than in hepatocytes. A second major conclusion is that no clear quantitative nutritional effects on the desaturation/elongation and β−oxidation activities in either hepatocytes or enterocytes of sea bass were observed upon the inclusion of vegetable oils in the diet.
Type: Journal Article
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Rights: Published in Aquaculture by Elsevier. Aquaculture, Volume 248, Issues 1-4, July 2005, pp. 173 - 186; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, VOL 248, ISSUE 1-4, (July 2005). DOI 10.1016/j.aquaculture.2005.04.023.
Notes: Aquaculture, Volume 248, Issues 1-4, Pages 1-340 (29 July 2005). Fish Nutrition and Feeding - Proceedings of the 11th International Symposium on Nutrition and Feeding in Fish organized by the Department of Fisheries, Thailand held in Phuket Island, Thailand, 2-7 May 2004. Edited by R.P. Wilson and M. Boonyaratpalin.
Affiliation: University of Cadiz

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