Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2813
Full metadata record
DC FieldValueLanguage
dc.contributor.authorStubhaug, Ingunnen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorTorstensen, Bente Een_UK
dc.date.accessioned2013-10-08T02:28:08Z-
dc.date.available2013-10-08T02:28:08Z-
dc.date.issued2005-06-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2813-
dc.description.abstractIsolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven 14C-labelled fatty acids. The fatty acids were; [1-14C]16:0, [1-14C]18:1n-9, [1-14C]18:2n-6, [1-14C]18:3n-3, [1-14C]20:4n-6, [1-14C]20:5n-3, and [1-14C]22:6n-3. After 2 hours of incubation the hepatocytes and medium were analyzed for acid soluble products, incorporation into lipid classes, and hepatocytes for desaturation and elongation. Uptake into hepatocytes was highest with [1-14C]18:2n-6 and [1-14C]20:5n-3 and lowest with [1-14C]16:0. The highest recovery of radioactivity in the cells was found in triacylglycerols. Of the phospholipids the highest recovery was found in phosphatidylcholine, with [1-14C]16:0 and [1-14C]22:6n-3 being the most prominent fatty acids. The rates of β-oxidation were as follows: 20:4n-6 > 18:2n-6 = 16:0 > 18:1n-9 > 22:6n-3 = 18:3n-3 = 20:5n-3. Of the fatty acids taken up by the hepatocytes, [1-14C]16:0 and [1-14C]18:1n-9 were subsequently exported the most, with the majority of radioactivity recovered in phospholipids and triacylglycerols, respectively. The major products from desaturation and elongation were generally one cycle of elongation of the fatty acids. Diet had a clear effect on the overall lipid metabolism, with replacing 75% of the fish oil with vegetable oil resulting in decreased uptake of all fatty acids and reduced incorporation of fatty acids into cellular lipids, but increased β-oxidation activity, and higher recovery in products of desaturation and elongation of [1-14C]18:2n-6 and [1-14C]18:3n-3.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationStubhaug I, Tocher DR, Bell JG, Dick JR & Torstensen BE (2005) Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1734 (3), pp. 277-288. https://doi.org/10.1016/j.bbalip.2005.04.003en_UK
dc.rightsPublished in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1734, Issue 3, June 2005, pp. 277 - 288.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1734, ISSUE 3, (June 2005). DOI 10.1016/j.bbalip.2005.04.003.en_UK
dc.subjectAtlantic salmonen_UK
dc.subjectSalmo salaren_UK
dc.subjectfish oilen_UK
dc.subjectvegetable oilen_UK
dc.subjectHepatocytesen_UK
dc.subjectLipid metabolismen_UK
dc.subjectFatty acid metabolismen_UK
dc.subjectFatty acids Metabolismen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectFishes Fooden_UK
dc.subjectVegetable oilsen_UK
dc.titleFatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oilen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.bbalip.2005.04.003en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1734en_UK
dc.citation.issue3en_UK
dc.citation.spage277en_UK
dc.citation.epage288en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date13/05/2005en_UK
dc.contributor.affiliationNational Institute of Nutrition and Seafood Research (NIFES)en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNational Institute of Nutrition and Seafood Research (NIFES)en_UK
dc.identifier.isiWOS:000230060900007en_UK
dc.identifier.scopusid2-s2.0-20444367639en_UK
dc.identifier.wtid838896en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2005-05-13en_UK
dc.date.filedepositdate2011-03-17en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorStubhaug, Ingunn|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorTorstensen, Bente E|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-03-17en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-03-17|en_UK
local.rioxx.filenameStubhaug et al final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1388-1981en_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Stubhaug et al final.pdfFulltext - Accepted Version1.1 MBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.