Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2810
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dc.contributor.authorMohd-Yusof, Nurul Yuzianaen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorMohd-Adnan, Aduraen_UK
dc.contributor.authorWan, Kiew-Lianen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2013-06-09T05:48:28Z-
dc.date.available2013-06-09T05:48:28Z-
dc.date.issued2010-12en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2810-
dc.description.abstractLates calcarifer, commonly known as the Asian sea bass or barramundi, is an interesting species that has great aquaculture potential in Asia including Malaysia and also Australia. We have investigated essential fatty acid metabolism in this species, focusing on the endogenous highly unsaturated fatty acid (HUFA) synthesis pathway using both biochemical and molecular biological approaches. Fatty acyl desaturase (Fad) and elongase (Elovl) cDNAs were cloned and functional characterization identified them as ∆6 Fad and Elovl5 elongase enzymes, respectively. The ∆6 Fad was equally active towards 18:3n-3 and 18:2n-6, and Elovl5 exhibited elongation activity for C18-20 and C20-22 elongation and a trace of C22-24 activity. The tissue profile of gene expression for ∆6 fad and elovl5 genes, showed brain to have the highest expression of both genes compared to all other tissues. The results of tissue fatty acid analysis showed that the brain contained more docosahexaenoic acid (DHA, 22:6n-3) than flesh, liver and intestine. The HUFA synthesis activity in isolated hepatocytes and enterocytes using [1-14C]18:3n-3 as substrate was very low with the only desaturated product detected being 18:4n-3. These findings indicate that L. calcarifer display an essential fatty acid pattern similar to other marine fish in that they appear unable to synthesize HUFA from C18 substrates. High expression of ∆6 fad and elovl5 genes in brain may indicate a role for these enzymes in maintaining high DHA levels in neural tissues through conversion of 20:5n-3.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationMohd-Yusof NY, Monroig O, Mohd-Adnan A, Wan K & Tocher DR (2010) Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcarifer. Fish Physiology and Biochemistry, 36 (4), pp. 827-843. http://www.springerlink.com/content/16l47312j325p15g/; https://doi.org/10.1007/s10695-010-9409-4en_UK
dc.rightsPublished in Fish Physiology and Biochemistry by Springer.; The original publication is available at www.springerlink.comen_UK
dc.subjectFatty aciden_UK
dc.subjectmetabolismen_UK
dc.subjectfatty acyl desaturaseen_UK
dc.subjectelongaseen_UK
dc.subjectbarramundien_UK
dc.subjectAsian sea bassen_UK
dc.subjectcDNAen_UK
dc.subjectfunctional characterisationen_UK
dc.subjectGiant perchen_UK
dc.subjectFatty acids Metabolismen_UK
dc.titleInvestigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcariferen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1007/s10695-010-9409-4en_UK
dc.citation.jtitleFish Physiology and Biochemistryen_UK
dc.citation.issn1573-5168en_UK
dc.citation.issn0920-1742en_UK
dc.citation.volume36en_UK
dc.citation.issue4en_UK
dc.citation.spage827en_UK
dc.citation.epage843en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.springerlink.com/content/16l47312j325p15g/en_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.contributor.affiliationNational University of Malaysiaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNational University of Malaysiaen_UK
dc.contributor.affiliationNational University of Malaysiaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000285069700002en_UK
dc.identifier.scopusid2-s2.0-78149498366en_UK
dc.identifier.wtid838791en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2010-12-31en_UK
dc.date.filedepositdate2011-03-17en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMohd-Yusof, Nurul Yuziana|en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorMohd-Adnan, Adura|en_UK
local.rioxx.authorWan, Kiew-Lian|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-03-17en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-03-17|en_UK
local.rioxx.filenameAsian sea bass(FISH663).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0920-1742en_UK
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