Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2805
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
Authors: Monroig, Oscar
Rotllant, Josep
Cerda-Reverter, Jose M
Dick, James R
Figueras, Antonio
Tocher, Douglas R
Contact Email: drt1@stir.ac.uk
Keywords: long-chain polyunsaturated fatty acids
elovl4 elongase
zebrafish
Danio rerio
embryonic development
whole-mount in situ hybridisation
gene expression
very long chain fatty acids
Issue Date: Oct-2010
Publisher: Elsevier
Citation: Monroig O, Rotllant J, Cerda-Reverter JM, Dick JR, Figueras A & Tocher DR (2010) Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1801 (10), pp. 1145-1154.
Abstract: Elovl4 is a fatty acyl elongase that participates in the biosynthesis of very long-chain fatty acids (≥C24), which are relatively abundant in skin (saturated chains), or retina, brain and testes (polyunsaturated chains) of mammals. In the present study we characterised two Elovl4 proteins, Elovl4a and Elovl4b, from zebrafish Danio rerio, and investigated their expression patterns during embryonic development. Heterologous expression in baker’s yeast showed that both zebrafish Elovl4 proteins efficiently elongated saturated fatty acids up to C36, with 26:0 appearing the preferred substrate as reported for human ELOVL4. Interestingly, activity for the elongation of PUFA substrates was only shown by Elovl4b, which effectively converted eicosapentaenoic (20:5n-3) and arachidonic (20:4n-6) acids to elongated polyenoic products up to C36. Furthermore, zebrafish Elovl4b may be involved in the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) as it had the capacity to elongate 22:5n-3 to 24:5n-3 which can be subsequently desaturated and chain shortened to DHA in peroxisomes. The distinct functional roles of zebrafish Elovl4 proteins were also reflected in their spatial-temporal expression patterns during ontogeny. Analyses by whole-mount in situ hybridisation in zebrafish embryos showed that elovl4a was expressed in neuronal tissues (wide-spread distribution in the head area), with elovl4b specifically expressed in epiphysis (pineal gland) and photoreceptor cells in the retina. Similarly, tissue distribution in adults revealed that elovl4a transcripts were found in most tissues analysed, whereas elovl4b expression was essentially restricted to eye and gonads. Overall, the results suggest that zebrafish elovl4b resembles other mammalian orthologues in terms of function and expression patterns, whereas elovl4a may represent an alternative elongase not previously described in vertebrates.
Type: Journal Article
URI: http://hdl.handle.net/1893/2805
URL: http://www.sciencedirect.com/science/journal/13881981
DOI Link: http://dx.doi.org/10.1016/j.bbalip.2010.06.005
Rights: Published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1801, Issue 10, October 2010, pp. 1145 - 1154; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1801, ISSUE 10, October 2010. DOI 10.1016/j.bbalip.2010.06.005
Affiliation: Aquaculture
Spanish National Research Council (CSIC)
Institute of Aquaculture Torre de la Sal
Aquaculture
Spanish National Research Council (CSIC)
Aquaculture

Files in This Item:
File Description SizeFormat 
Zebrafish elovl4 final.pdf9.1 MBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.