|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Molecular cloning, characterization and nutritional regulation of key enzymes required for the effective utilization of marine wax esters by Atlantic salmon (Salmo salar L.)|
Olsen, Rolf E
Tocher, Douglas R
fatty alcohol dehydrogenase
fatty aldehyde dehydrogenase
|Citation:||Minghetti M, Olsen RE & Tocher DR (2010) Molecular cloning, characterization and nutritional regulation of key enzymes required for the effective utilization of marine wax esters by Atlantic salmon (Salmo salar L.), Aquaculture Nutrition, 16 (5), pp. 483-495.|
|Abstract:||Previous studies had shown that wax ester-rich lipid extracted from calanoid copepods could be a useful alternative to fish oil as a provider of long chain n-3 polyunsaturated fatty acids in diets for use in salmon aquaculture. Effective utilization of wax ester requires digestion and metabolism in the intestine with the fatty alcohol component being oxidized to fatty acid in intestinal cells through the combined activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). We studied wax ester utilization in Atlantic salmon using a candidate gene approach, focusing on ADH and ALDH as sequence information was available for these genes, including fish sequences, facilitating isolation of the cDNAs. Here we report on the isolation and cloning of full-length cDNAs for ADH3 and ALDH3a2 genes from salmon intestinal tissue, and their functional characterisation by heterologous expression in the yeast, Saccharomyces cerevisiae. The tissue expression profile of the genes and whether their expression was nutritionally regulated in salmon fed dietary copepod oil in comparison to fish fed fish oil was determined by quantitative PCR (qPCR).|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.|
Matre Aquaculture Research Station
|Minghetti et al.pdf||562.58 kB||Adobe PDF||Under Embargo until 31/12/2999 Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.