Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2742
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Biosynthesis of long-chain polyunsaturated fatty acids in marine fish: Characterization of an Elovl4-like elongase from cobia Rachycentron canadum and activation of the pathway during early life stages
Authors: Monroig, Oscar
Webb, Ken
Ibarra-Castro, Leonardo
Holt, G Joan
Tocher, Douglas R
Contact Email: drt1@stir.ac.uk
Keywords: Cobia
Rachycentron canadum
Polyunsaturated fatty acid
Biosynthesis
Genes
Elovl4 elongase
cDNA
Cloning
Functional characterisation
Tissue distribution
Larval development
Issue Date: 25-Feb-2011
Publisher: Elsevier
Citation: Monroig O, Webb K, Ibarra-Castro L, Holt GJ & Tocher DR (2011) Biosynthesis of long-chain polyunsaturated fatty acids in marine fish: Characterization of an Elovl4-like elongase from cobia Rachycentron canadum and activation of the pathway during early life stages, Aquaculture, 312 (1-4), pp. 145-153.
Abstract: Marine fish, unlike freshwater species, have been generally considered to have a limited ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C18 precursors due to apparent limited enzymatic activities involved in the pathway. Although LC-PUFA play important physiological roles throughout the entire life cycle, requirements for early life stages are especially high and provision of preformed LC-PUFA in egg lipids appears critical to support the formation of developing tissues where these compounds accumulate. No studies, however, have been conducted to explore the capability of marine fish embryos (here referring to life stages from zygote to the oesophagus opening) for de novo synthesis of the LC-PUFA required for normal growth and development. The present study aimed to investigate the activation of the LC-PUFA biosynthetic pathway during embryogenesis of the marine teleost cobia (Rachycentron canadum). First, a fatty acyl elongase with sequence similarity to mammalian elongase of very long-chain fatty acids 4 (Elovl4) was isolated, and its biochemical function characterized showing that it catalyzed the production of very long-chain fatty acids (VLC-FA) including both saturated and polyunsaturated fatty acids with chain lenghts ≥ 24 carbons. Notably, cobia Elovl4 was able to elongate 22:5n-3 to 24:5n-3 and thus could play a key role in the biosynthesis of docosahexaenoic acid (22:6n-3), a critical fatty acid in neural tissues. Subsequently, the fatty acid dynamics of embryos at different developmental stages and the temporal expression patterns of target genes including elovl4, and the formerly characterized elovl5 elongase and ∆6 fatty acyl desaturase, were analyzed in order to elucidate the overall activation of the LC-PUFA biosynthetic pathway in cobia embryos. Our results indicated that expression of the LC-PUFA biosynthetic pathway in cobia embryos is initiated at 12-18 hours post-fertilization.
Type: Journal Article
URI: http://hdl.handle.net/1893/2742
URL: http://www.sciencedirect.com/science/journal/00448486
DOI Link: http://dx.doi.org/10.1016/j.aquaculture.2010.12.024
Rights: Published in Aquaculture by Elsevier.; Aquaculture, Volume 312, Issue 1-4, February 2011, pp. 145 - 153
Affiliation: Aquaculture
University of Texas At Austin
University of Texas At Austin
University of Texas At Austin
Aquaculture

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