Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27001
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Life‐stage‐associated remodelling of lipid metabolism regulation in Atlantic salmon
Authors: Gillard, Gareth
Harvey, Thomas
Gjuvsland, Arne
Jin, Yang
Thomassen, Magny
Lien, Sigbjorn
Leaver, Michael
Torgerson, Jacob
Hvidsten, Torgeir
Vik, Jon Olav
Sandve, Simen
Contact Email: mjl1@stir.ac.uk
Keywords: adaptation
fish
life stage
metabolism
transcriptomics
Issue Date: Mar-2018
Citation: Gillard G, Harvey T, Gjuvsland A, Jin Y, Thomassen M, Lien S, Leaver M, Torgerson J, Hvidsten T, Vik JO & Sandve S (2018) Life‐stage‐associated remodelling of lipid metabolism regulation in Atlantic salmon, Molecular Ecology, 27 (5), pp. 1200-1213.
Abstract: Atlantic salmon migrates from rivers to sea to feed, grow and develop gonads before returning to spawn in freshwater. The transition to marine habitats is associated with dramatic changes in the environment, including water salinity, exposure to pathogens and shift in dietary lipid availability. Many changes in physiology and metabolism occur across this life‐stage transition, but little is known about the molecular nature of these changes. Here, we use a long‐term feeding experiment to study transcriptional regulation of lipid metabolism in Atlantic salmon gut and liver in both fresh‐ and saltwater. We find that lipid metabolism becomes significantly less plastic to differences in dietary lipid composition when salmon transitions to saltwater and experiences increased dietary lipid availability. Expression of genes in liver relating to lipogenesis and lipid transport decreases overall and becomes less responsive to diet, while genes for lipid uptake in gut become more highly expressed. Finally, analyses of evolutionary consequences of the salmonid‐specific whole‐genome duplication on lipid metabolism reveal several pathways with significantly different (p<.05) duplicate retention or duplicate regulatory conservation. We also find a limited number of cases where the whole‐genome duplication has resulted in an increased gene dosage. In conclusion, we find variable and pathway‐specific effects of the salmonid genome duplication on lipid metabolism genes. A clear life‐stage‐associated shift in lipid metabolism regulation is evident, and we hypothesize this to be, at least partly, driven by nondietary factors such as the preparatory remodelling of gene regulation and physiology prior to sea migration.
DOI Link: http://dx.doi.org/10.1111/mec.14533
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Gillard G, Harvey TN, Gjuvsland A, et al. Life‐stage‐associated remodelling of lipid metabolism regulation in Atlantic salmon. Molecular Ecology 2018;27:1200–1213, which has been published in final form at https://doi.org/10.1111/mec.14533. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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