Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26059
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care
Authors: Torati, Lucas S
Migaud, Herve
Doherty, Mary K
Siwy, Justyna
Mullen, William
Mesquita, Pedro E C
Albalat, Amaya
Issue Date: 24-Oct-2017
Citation: Torati LS, Migaud H, Doherty MK, Siwy J, Mullen W, Mesquita PEC & Albalat A (2017) Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care, PLoS ONE, 12 (10), Art. No.: e0186692.
Abstract: Parental investment in Arapaima gigas includes nest building and guarding, followed by a care provision when a cephalic fluid is released from the parents’ head to the offspring. This fluid has presumably important functions for the offspring but so far its composition has not been characterised. In this study the proteome and peptidome of the cephalic secretion was studied in parental and non-parental fish using capillary electrophoresis coupled to mass spectrometry (CE-MS) and GeLC-MS/MS analyses. Multiple comparisons revealed 28 peptides were significantly different between males and parental males (PC-males), 126 between females and parental females (PC-females), 51 between males and females and 9 between PC-males and PC-females. Identification revealed peptides were produced in the inner ear (pcdh15b), eyes (tetraspanin and ppp2r3a), central nervous system (otud4, ribeye a, tjp1b and syn1) among others. A total of 422 proteins were also identified and gene ontology analysis revealed 28 secreted extracellular proteins. From these, 2 hormones (prolactin and stanniocalcin) and 12 proteins associated to immunological processes (serotransferrin, α-1-antitrypsin homolog, apolipoprotein A-I, and others) were identified. This study provides novel biochemical data on the lateral line fluid which will enable future hypotheses-driven experiments to better understand the physiological roles of the lateral line in chemical communication.
DOI Link: http://dx.doi.org/10.1371/journal.pone.0186692
Rights: © 2017 Torati et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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