Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25924
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dc.contributor.authorHodson, Nathanen_UK
dc.contributor.authorMcGlory, Chrisen_UK
dc.contributor.authorOikawa, Sara Yen_UK
dc.contributor.authorJeromson, Stewarten_UK
dc.contributor.authorSong, Zheen_UK
dc.contributor.authorRuegg, Markus Aen_UK
dc.contributor.authorHamilton, David Leeen_UK
dc.contributor.authorPhillips, Stuart Men_UK
dc.contributor.authorPhilp, Andrewen_UK
dc.date.accessioned2018-01-11T01:11:49Z-
dc.date.available2018-01-11T01:11:49Z-
dc.date.issued2017-12-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25924-
dc.description.abstractMechanistic target of rapamycin (mTOR) resides as two complexes within skeletal muscle. mTOR complex 1 (mTORC1-Raptor positive) regulates skeletal muscle growth, whereas mTORC2 (Rictor positive) regulates insulin sensitivity. To examine the regulation of these complexes in human skeletal muscle, we utilised immunohistochemical analysis to study the localisation of mTOR complexes prior to and following protein-carbohydrate feeding (FED) and resistance exercise plus protein-carbohydrate feeding (EXFED) in unilateral exercise model. In basal samples, mTOR and the lysosomal marker LAMP2 were highly co-localized and remained so throughout. In the FED and EXFED states, mTOR/LAMP2 complexes were redistributed to the cell periphery (WGA positive staining) (time effect; p=.025), with 39\% (FED) and 26\% (EXFED) increases in mTOR/WGA association observed 1h post-feeding/exercise. mTOR/WGA colocalisation continued to increase in EXFED at 3h (48\% above baseline) whereas colocalisation decreased in FED (21\% above baseline). A significant effect of condition (p=.05) was noted suggesting mTOR/WGA co-localization was greater during EXFED. This pattern was replicated in Raptor/WGA association, where a significant difference between EXFED and FED was noted at 3h post-exercise/feeding (p=.014). Rictor/WGA colocalization remained unaltered throughout the trial. Alterations in mTORC1 cellular location coincided with elevated S6K1 kinase activity, which rose to a greater extent in EXFED compared to FED at 1h post-exercise/feeding (p<.001), and only remained elevated in EXFED at the 3h time point (p=.037). Collectively these data suggest that mTORC1 redistribution within the cell is a fundamental response to resistance exercise and feeding, whereas mTORC2 is predominantly situated at the sarcolemma and does not alter localisation.en_UK
dc.language.isoenen_UK
dc.publisherAmerican Physiological Societyen_UK
dc.relationHodson N, McGlory C, Oikawa SY, Jeromson S, Song Z, Ruegg MA, Hamilton DL, Phillips SM & Philp A (2017) Differential localization and anabolic responsiveness of mTOR complexes in human skeletal muscle in response to feeding and exercise. American Journal of Physiology - Cell Physiology, 313 (6), pp. C604-C611. https://doi.org/10.1152/ajpcell.00176.2017en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository. Published in American Journal of Physiology - Cell Physiology by American Physiological Society. The version of record is available at: http://www.physiology.org/doi/abs/10.1152/ajpcell.00176.2017en_UK
dc.titleDifferential localization and anabolic responsiveness of mTOR complexes in human skeletal muscle in response to feeding and exerciseen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-11-28en_UK
dc.rights.embargoreason[Hodson-et-al-17.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1152/ajpcell.00176.2017en_UK
dc.identifier.pmid28971834en_UK
dc.citation.jtitleAmerican Journal of Physiology - Cell Physiologyen_UK
dc.citation.issn1522-1563en_UK
dc.citation.issn0363-6143en_UK
dc.citation.volume313en_UK
dc.citation.issue6en_UK
dc.citation.spageC604en_UK
dc.citation.epageC611en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderSociety for Endocrinologyen_UK
dc.author.emaild.l.hamilton@stir.ac.uken_UK
dc.citation.date27/09/2017en_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationMcMaster Universityen_UK
dc.contributor.affiliationMcMaster Universityen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Baselen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationMcMaster Universityen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.identifier.isiWOS:000417614100002en_UK
dc.identifier.scopusid2-s2.0-85036667935en_UK
dc.identifier.wtid517912en_UK
dc.contributor.orcid0000-0002-5620-4788en_UK
dc.date.accepted2017-09-23en_UK
dcterms.dateAccepted2017-09-23en_UK
dc.date.filedepositdate2017-09-28en_UK
dc.relation.funderprojectDevelopment of a Dual Reporter for Insulin and Anabolic Resistanceen_UK
dc.relation.funderrefEarly career granten_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorHodson, Nathan|en_UK
local.rioxx.authorMcGlory, Chris|en_UK
local.rioxx.authorOikawa, Sara Y|en_UK
local.rioxx.authorJeromson, Stewart|en_UK
local.rioxx.authorSong, Zhe|en_UK
local.rioxx.authorRuegg, Markus A|en_UK
local.rioxx.authorHamilton, David Lee|0000-0002-5620-4788en_UK
local.rioxx.authorPhillips, Stuart M|en_UK
local.rioxx.authorPhilp, Andrew|en_UK
local.rioxx.projectEarly career grant|Society for Endocrinology|http://dx.doi.org/10.13039/501100000382en_UK
local.rioxx.freetoreaddate2018-11-28en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-11-27en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2018-11-28|en_UK
local.rioxx.filenameHodson-et-al-17.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0363-6143en_UK
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