Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25805
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom (Forthcoming)
Authors: Ngo, Thao P H
Smith, Peter
Bartie, Kerry
Thompson, Kim D
Verner-Jeffreys, David W
Hoare, Rowena
Adams, Alexandra
Contact Email: rowena.hoare1@stir.ac.uk
Keywords: Flavobacterium psychrophilum
antimicrobial susceptibility
epidemiological cut-off values
disc diffusion
broth microdilution
rainbow trout fry syndrome
Citation: Ngo TPH, Smith P, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2017) Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom (Forthcoming), Journal of Fish Diseases.
Abstract: Routine application of antimicrobials is the current treatment of choice for rainbow trout fry syndrome (RTFS) or bacterial coldwater disease (BCWD) caused by Flavobacterium psychrophilum. In this study, the antimicrobial susceptibilities of 133 F. psychrophilum isolates, 118 of which were from the UK, were evaluated by broth microdilution and disc diffusion methods following VET04-A2 and VET03-A guidelines of Clinical and Laboratory Standards Institute (CLSI), respectively. Isolates were categorised as wild type (fully susceptible, WT) or non-wild type (NWT) using normalised resistance interpretation (NRI) determined cut-off values (COWT). Broth microdilution testing showed that only 12% of UK isolates were WT to oxolinic acid (MIC COWT ≤0.25 mg L-1) and 42% were WT for oxytetracycline (MIC COWT ≤0.25 mg L-1). In contrast, all the isolates tested were WT (MIC COWT ≤2 mg L-1) for florfenicol, the main antimicrobial for RTFS control in the UK. Disc diffusion-based COWT values were ≥51 mm for 10 µg amoxicillin, ≥44 mm for 30 µg florfenicol, ≥30 mm for 2 µg oxolinic acid and ≥51 mm for 30 µg oxytetracycline. There was a high categorical agreement between the classifications of the isolates by two testing methods for florfenicol (100%), oxytetracycline (93%), and oxolinic acid (99%).
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