Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25706
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Subcellular localization and function study of a secreted phospholipase C from Nocardia seriolae (Forthcoming/Available Online)
Authors: Xia, Liqin
Liang, Haiying
Xu, Liang
Chen, Jianlin
Bekaert, Michaël
Zhang, Honglian
Yu, Lishan
Contact Email: michael.bekaert@stir.ac.uk
Keywords: Nocardia seriolae
phospholipase C
subcellular localization
secreted protein
cell apoptosis
overexpression
Issue Date: 7-Jul-2017
Citation: Xia L, Liang H, Xu L, Chen J, Bekaert M, Zhang H & Yu L (2017) Subcellular localization and function study of a secreted phospholipase C from Nocardia seriolae (Forthcoming/Available Online), FEMS Microbiology Letters.
Abstract: Fish nocardiosis is a chronic systemic granulomatous disease, andNocardia seriolaeis the main pathogen that causes this disease. But the pathogenesis and virulence factors ofN. seriolaeare not fully understood. A phospholipase C (PLC), which was likely to be a secreted protein targeting host cell mitochondria, was found by the bioinformatics analysis on the whole genome sequence ofN. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC fromN. seriolae(NsPLC), the gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out in this study. The results showed that NsPLC was a secreted protein by mass spectrometry analysis of extracellular products fromN. seriolae. Subcellular localization of NsPLC-GFP fusion protein in FHM cells revealed that the green fluorescence exhibited a punctate distribution near the nucleus and did not co-localize with mitochondria. In addition, apoptosis assay suggested that apoptosis was induced in FHM cells by the overexpression of NsPLC. This study may lay the foundation for further study on the function of NsPLC and promote the understanding of the virulence factors and pathogenic mechanism ofN. seriolae.
DOI Link: http://dx.doi.org/10.1093/femsle/fnx143
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.

Files in This Item:
File Description SizeFormat 
fnx143.pdf592.16 kBAdobe PDFUnder Embargo until 8/7/2018     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.