Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25686
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dc.contributor.authorSong, Zheen_UK
dc.contributor.authorMoore, Daniel Ren_UK
dc.contributor.authorHodson, Nathanen_UK
dc.contributor.authorWard, Carlen_UK
dc.contributor.authorDent, Jessica Ren_UK
dc.contributor.authorO'Leary, Mary Fen_UK
dc.contributor.authorShaw, Andyen_UK
dc.contributor.authorHamilton, David Leeen_UK
dc.contributor.authorSarkar, Sovanen_UK
dc.contributor.authorGangloff, Yann-Gaelen_UK
dc.contributor.authorHornberger, Troy Aen_UK
dc.contributor.authorSpriet, Lawrence Len_UK
dc.contributor.authorHeigenhauser, George J Fen_UK
dc.contributor.authorPhilp, Andrewen_UK
dc.date.accessioned2017-08-04T22:12:17Z-
dc.date.available2017-08-04T22:12:17Z-
dc.date.issued2017-07-10en_UK
dc.identifier.other14en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25686-
dc.description.abstractThe mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.en_UK
dc.language.isoenen_UK
dc.publisherSpringer Natureen_UK
dc.relationSong Z, Moore DR, Hodson N, Ward C, Dent JR, O'Leary MF, Shaw A, Hamilton DL, Sarkar S, Gangloff Y, Hornberger TA, Spriet LL, Heigenhauser GJF & Philp A (2017) Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle. Scientific Reports, 7, Art. No.: 14. https://doi.org/10.1038/s41598-017-05483-xen_UK
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectcell biologyen_UK
dc.subjectTOR signallingen_UK
dc.titleResistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscleen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1038/s41598-017-05483-xen_UK
dc.identifier.pmid28694500en_UK
dc.citation.jtitleScientific Reportsen_UK
dc.citation.issn2045-2322en_UK
dc.citation.volume7en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.citation.date10/07/2017en_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Torontoen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversite Lyon, Franceen_UK
dc.contributor.affiliationUniversity of Wisconsin-Madisonen_UK
dc.contributor.affiliationUniversity of Guelphen_UK
dc.contributor.affiliationMcMaster Universityen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.identifier.isiWOS:000405180900101en_UK
dc.identifier.scopusid2-s2.0-85022345085en_UK
dc.identifier.wtid522486en_UK
dc.contributor.orcid0000-0002-5620-4788en_UK
dc.date.accepted2017-06-19en_UK
dcterms.dateAccepted2017-06-19en_UK
dc.date.filedepositdate2017-08-04en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSong, Zhe|en_UK
local.rioxx.authorMoore, Daniel R|en_UK
local.rioxx.authorHodson, Nathan|en_UK
local.rioxx.authorWard, Carl|en_UK
local.rioxx.authorDent, Jessica R|en_UK
local.rioxx.authorO'Leary, Mary F|en_UK
local.rioxx.authorShaw, Andy|en_UK
local.rioxx.authorHamilton, David Lee|0000-0002-5620-4788en_UK
local.rioxx.authorSarkar, Sovan|en_UK
local.rioxx.authorGangloff, Yann-Gael|en_UK
local.rioxx.authorHornberger, Troy A|en_UK
local.rioxx.authorSpriet, Lawrence L|en_UK
local.rioxx.authorHeigenhauser, George J F|en_UK
local.rioxx.authorPhilp, Andrew|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-08-04en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-08-04|en_UK
local.rioxx.filenameSong_et_al-2017-Scientific_Reports.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source2045-2322en_UK
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