Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25684
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dc.contributor.authorSbirkov, Yordanen_UK
dc.contributor.authorKwok, Colinen_UK
dc.contributor.authorBhamra, Amandeepen_UK
dc.contributor.authorThompson, Andrew Jen_UK
dc.contributor.authorGil, Veronicaen_UK
dc.contributor.authorZelent, Arthuren_UK
dc.contributor.authorPetrie, Kevinen_UK
dc.date.accessioned2017-09-23T02:03:03Z-
dc.date.available2017-09-23T02:03:03Z-
dc.date.issued2017-07-05en_UK
dc.identifier.other1440en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25684-
dc.description.abstractAlterations to the gene encoding the EZH2 (KMT6A) methyltransferase, including both gain-of-function and loss-of-function, have been linked to a variety of haematological malignancies and solid tumours, suggesting a complex, context-dependent role of this methyltransferase. The successful implementation of molecularly targeted therapies against EZH2 requires a greater understanding of the potential mechanisms by which EZH2 contributes to cancer. One aspect of this effort is the mapping of EZH2 partner proteins and cellular targets. To this end we performed affinity-purification mass spectrometry in the FAB-M2 HL-60 acute myeloid leukaemia (AML) cell line before and after all-transretinoic acid-induced differentiation. These studies identified new EZH2 interaction partners and potential non-histone substrates for EZH2-mediated methylation. Our results suggest that EZH2 is involved in the regulation of translation through interactions with a number of RNA binding proteins and by methylating key components of protein synthesis such as eEF1A1. Given that deregulated mRNA translation is a frequent feature of cancer and that eEF1A1 is highly expressed in many human tumours, these findings present new possibilities for the therapeutic targeting of EZH2 in AML.en_UK
dc.language.isoenen_UK
dc.publisherMDPIen_UK
dc.relationSbirkov Y, Kwok C, Bhamra A, Thompson AJ, Gil V, Zelent A & Petrie K (2017) Semi-quantitative mass spectrometry in AML cells identifies new non-genomic targets of the EZH2 methyltransferase. International Journal of Molecular Sciences, 18 (7), Art. No.: 1440. https://doi.org/10.3390/ijms18071440en_UK
dc.rights© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectacute myeloid leukaemiaen_UK
dc.subjectEZH2en_UK
dc.subjectmass spectrometryen_UK
dc.subjectmethylationen_UK
dc.subjecteEF1A1en_UK
dc.titleSemi-quantitative mass spectrometry in AML cells identifies new non-genomic targets of the EZH2 methyltransferaseen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.3390/ijms18071440en_UK
dc.identifier.pmid28678185en_UK
dc.citation.jtitleInternational Journal of Molecular Sciencesen_UK
dc.citation.issn1422-0067en_UK
dc.citation.issn1661-6596en_UK
dc.citation.volume18en_UK
dc.citation.issue7en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.citation.date05/07/2017en_UK
dc.contributor.affiliationJulius Maximilians University of Wurzburgen_UK
dc.contributor.affiliationInstitute of Cancer Researchen_UK
dc.contributor.affiliationUniversity College Londonen_UK
dc.contributor.affiliationInstitute of Cancer Researchen_UK
dc.contributor.affiliationInstitute of Cancer Researchen_UK
dc.contributor.affiliationUniversity of Miami, USAen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.identifier.isiWOS:000408746800110en_UK
dc.identifier.scopusid2-s2.0-85022075761en_UK
dc.identifier.wtid522382en_UK
dc.contributor.orcid0000-0002-9805-9152en_UK
dc.date.accepted2017-06-29en_UK
dcterms.dateAccepted2017-06-29en_UK
dc.date.filedepositdate2017-08-03en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSbirkov, Yordan|en_UK
local.rioxx.authorKwok, Colin|en_UK
local.rioxx.authorBhamra, Amandeep|en_UK
local.rioxx.authorThompson, Andrew J|en_UK
local.rioxx.authorGil, Veronica|en_UK
local.rioxx.authorZelent, Arthur|en_UK
local.rioxx.authorPetrie, Kevin|0000-0002-9805-9152en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-08-03en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-08-03|en_UK
local.rioxx.filenameijms-18-01440.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1661-6596en_UK
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