Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25544
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays
Authors: Cruz, Vanessa P
Vera, Manuel
Pardo, Belen G
Taggart, John
Martinez, Paulino
Oliveira, Claudio
Foresti, Fausto
Contact Email: j.b.taggart@stir.ac.uk
Keywords: conservation tools
genetic fish
hybridization
NGS techniques
Potamotrygon
SNPs
Issue Date: May-2017
Citation: Cruz VP, Vera M, Pardo BG, Taggart J, Martinez P, Oliveira C & Foresti F (2017) Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays, Molecular Ecology Resources, 17 (3), pp. 550-556.
Abstract: Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226774 reads (65.5Mb) were obtained (mean read length 289±183bp) detecting a total of 5399 contigs (mean contig length: 396±91bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P.falkneri, 21 in P.motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.
DOI Link: http://dx.doi.org/10.1111/1755-0998.12564
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