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http://hdl.handle.net/1893/25544| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays |
| Author(s): | Cruz, Vanessa P Vera, Manuel Pardo, Belen G Taggart, John Martinez, Paulino Oliveira, Claudio Foresti, Fausto |
| Contact Email: | j.b.taggart@stir.ac.uk |
| Keywords: | conservation tools genetic fish hybridization NGS techniques Potamotrygon SNPs |
| Issue Date: | May-2017 |
| Date Deposited: | 26-Jun-2017 |
| Citation: | Cruz VP, Vera M, Pardo BG, Taggart J, Martinez P, Oliveira C & Foresti F (2017) Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays. Molecular Ecology Resources, 17 (3), pp. 550-556. https://doi.org/10.1111/1755-0998.12564 |
| Abstract: | Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226774 reads (65.5Mb) were obtained (mean read length 289±183bp) detecting a total of 5399 contigs (mean contig length: 396±91bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P.falkneri, 21 in P.motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection. |
| DOI Link: | 10.1111/1755-0998.12564 |
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| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
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|---|---|---|---|---|
| Cruz_et_al-2017-Molecular_Ecology_Resources.pdf | Fulltext - Published Version | 438.84 kB | Adobe PDF | Under Embargo until 2999-12-29 Request a copy |
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