Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25543
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dc.contributor.authorHerath, Tharanganien_UK
dc.contributor.authorAshby, Angela Jen_UK
dc.contributor.authorJayasuriya, Nilantha Sandaruwanen_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorTaylor, Johnen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorRichards, Randolphen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorFerguson, Hughen_UK
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.contributor.authorFordyce, Mark Jen_UK
dc.contributor.authorThompson, Kim Den_UK
dc.date.accessioned2017-10-20T01:08:54Z-
dc.date.available2017-10-20T01:08:54Z-
dc.date.issued2017-09-26en_UK
dc.identifier.othere0179192en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25543-
dc.description.abstractWith increasing interest in the use of triploid salmon in commercial aquaculture, gaining an understanding of how economically important pathogens affect triploid stocks is important. To compare the susceptibility of diploid and triploid Atlantic salmon (Salmo salar L.) to viral pathogens, fry were experimentally infected with Salmonid alphavirus sub-type 1 (SAV1), the aetiological agent of pancreas disease (PD) affecting Atlantic salmon aquaculture in Europe. Three groups of fry were exposed to the virus via different routes of infection: intraperitoneal injection (IP), bath immersion, or cohabitation (co-hab) and untreated fry were used as a control group. Mortalities commenced in the co-hab challenged diploid and triploid fish from 11 days post infection (dpi), and the experiment was terminated at 17 dpi. Both diploid and triploid IP challenged groups had similar levels of cumulative mortality at the end of the experimental period (41.1 % and 38.9 % respectively), and these were significantly higher (p < 0.01) than for the other challenge routes. A TaqMan-based quantitative PCR was used to assess SAV load in the heart, a main target organ of the virus, and also liver, which does not normally display any pathological changes during clinical infections, but exhibited severe degenerative lesions in the present study. The median viral RNA copy number was higher in diploid fish compared to triploid fish in both the heart and the liver of all three challenged groups. However, a significant statistical difference (p < 0.05) was only apparent in the liver of the co-hab groups. Diploid fry also displayed significantly higher levels of pancreatic and myocardial degeneration than triploids. This study showed that both diploid and triploid fry are susceptible to experimental SAV1 infection. The lower virus load seen in the triploids compared to the diploids may possibly be related to differences in cell metabolism between the two groups, however, further investigation is necessary to confirm this and also to assess the outcome of PD outbreaks in other developmental stages of the fish when maintained in commercial production systems.en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationHerath T, Ashby AJ, Jayasuriya NS, Bron J, Taylor J, Adams A, Richards R, Weidmann M, Ferguson H, Taggart J, Migaud H, Fordyce MJ & Thompson KD (2017) Impact of Salmonid alphavirus infection in diploid and triploid Atlantic salmon (Salmo salar L.) fry. PLoS ONE, 12 (9), Art. No.: e0179192. https://doi.org/10.1371/journal.pone.0179192en_UK
dc.rights© 2017 Herath et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectSalmonen_UK
dc.subjectsalmonid alphavirusen_UK
dc.subjecttriploidsen_UK
dc.subjectdiploidsen_UK
dc.subjectfryen_UK
dc.titleImpact of Salmonid alphavirus infection in diploid and triploid Atlantic salmon (Salmo salar L.) fryen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/journal.pone.0179192en_UK
dc.identifier.pmid28949966en_UK
dc.citation.jtitlePLoS ONEen_UK
dc.citation.issn1932-6203en_UK
dc.citation.volume12en_UK
dc.citation.issue9en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.citation.date26/09/2017en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationScottish Government - Enterprise, Environment & Digital - Marine Scotlanden_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.identifier.isiWOS:000411671000001en_UK
dc.identifier.scopusid2-s2.0-85029937984en_UK
dc.identifier.wtid525648en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.contributor.orcid0000-0003-4370-7922en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.date.accepted2017-05-25en_UK
dcterms.dateAccepted2017-05-25en_UK
dc.date.filedepositdate2017-06-26en_UK
rioxxterms.apcpaiden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorHerath, Tharangani|en_UK
local.rioxx.authorAshby, Angela J|en_UK
local.rioxx.authorJayasuriya, Nilantha Sandaruwan|en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorTaylor, John|0000-0003-4370-7922en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.authorRichards, Randolph|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorFerguson, Hugh|en_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.authorFordyce, Mark J|en_UK
local.rioxx.authorThompson, Kim D|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-09-26en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2017-09-26en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-09-26|en_UK
local.rioxx.filenamejournal.pone.0179192.pdfen_UK
local.rioxx.filecount1en_UK
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