Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25535
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dc.contributor.authorBenitez-Dorta, Vanessaen_UK
dc.contributor.authorCaballero, Maria Joseen_UK
dc.contributor.authorBetancor, Monicaen_UK
dc.contributor.authorManchado, Manuelen_UK
dc.contributor.authorTort, Lluisen_UK
dc.contributor.authorTorrecillas, Silviaen_UK
dc.contributor.authorZamorano, Maria Jen_UK
dc.contributor.authorIzquierdo, Marisol Sen_UK
dc.contributor.authorMontero, Danielen_UK
dc.date.accessioned2017-09-23T02:10:13Z-
dc.date.available2017-09-23T02:10:13Z-
dc.date.issued2017-10-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25535-
dc.description.abstractThe present study examined the short and mid-term effects of a rise in temperature from 18 ºC to 24 ºC on the expression of genes related to the stress response regulation in juveniles of Senegalese sole, Solea senegalensis. The animals were exposed to a temperature increase of 6 °C, after 1 month of acclimation at 18 ºC. After this process, samples of different tissues were collected from a total of 96 fish at four sampling points: 1 hour, 24 hours, 3 days and 1 week. The transcript levels of a set of genes involved in the stress response such as glucocorticoid receptors 1 and 2, corticotrophin-releasing factor, corticotrophin-releasing factor binding proteins, proopiomelanocortin A and B, and cellular stress defense (heat shock protein 70, 90AA and 90AB) were quantified at these sampling points. Additionally, blood samples were also taken to measure the circulating plasma cortisol concentration.  Thermal stress induced by increasing temperature prompted an elevation of plasma cortisol levels in juvenile Senegalese sole after 1 h as a short-term response, and a consecutive increase after one week, as a mid-term response.. Senegalese sole seemed to respond positively in terms of adaptive mechanisms, with a rapid over-expression of grs and hsps in liver and brain, significantly higher after one hour post stress, denoting the fast and acute response of those tissues to a rapid change on temperature. The ratio hsp90/gr also increased 24 h after thermal shock, ratio proposed to be an adaptive mechanism to prevent proteosomal degradation of GR. As a mid-term response, the elevation of brain crfbp gene expression one week after thermal shock could be an adaptive mechanism of negative feedback on HPI axis Taken together, these data suggested an initial up-regulation of the glucocorticoid receptor complex linked genes in response to a temperature increase in Senegalese sole, with heat shock protein 90 potentially being a regulatory factor for the glucocorticoid receptor in the presence of cortisol.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationBenitez-Dorta V, Caballero MJ, Betancor M, Manchado M, Tort L, Torrecillas S, Zamorano MJ, Izquierdo MS & Montero D (2017) Effects of thermal stress on the expression of glucocorticoid receptor complex linked genes in Senegalese sole (Solea senegalensis): Acute and adaptive stress responses. General and Comparative Endocrinology, 252, pp. 173-185. https://doi.org/10.1016/j.ygcen.2017.06.022en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Benitez-Dorta V, Caballero MJ, Betancor M, Manchado M, Tort L, Torrecillas S, Zamorano MJ, Izquierdo MS & Montero D (2017) Effects of thermal stress on the expression of glucocorticoid receptor complex linked genes in Senegalese sole (Solea senegalensis): Acute and adaptive stress responses, General and Comparative Endocrinology, 252, pp. 173-185. DOI: 10.1016/j.ygcen.2017.06.022 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectCortisolen_UK
dc.subjectTemperatureen_UK
dc.subjectGlucocorticoid receptoren_UK
dc.subjectHeat shock proteinen_UK
dc.subjectProopiomelanocortinen_UK
dc.subjectCorticotrophin-releasing factoren_UK
dc.subjectCorticotrophin-releasing factor binding proteinsen_UK
dc.titleEffects of thermal stress on the expression of glucocorticoid receptor complex linked genes in Senegalese sole (Solea senegalensis): Acute and adaptive stress responsesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-06-24en_UK
dc.rights.embargoreason[Benitez et al 2017.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.ygcen.2017.06.022en_UK
dc.identifier.pmid28652134en_UK
dc.citation.jtitleGeneral and Comparative Endocrinologyen_UK
dc.citation.issn0016-6480en_UK
dc.citation.volume252en_UK
dc.citation.spage173en_UK
dc.citation.epage185en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailmbb4@stir.ac.uken_UK
dc.citation.date23/06/2017en_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationIFAPA Centre El Toruñoen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.identifier.isiWOS:000409396400018en_UK
dc.identifier.scopusid2-s2.0-85021381917en_UK
dc.identifier.wtid525723en_UK
dc.contributor.orcid0000-0003-1626-7458en_UK
dc.date.accepted2017-06-22en_UK
dcterms.dateAccepted2017-06-22en_UK
dc.date.filedepositdate2017-06-25en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorBenitez-Dorta, Vanessa|en_UK
local.rioxx.authorCaballero, Maria Jose|en_UK
local.rioxx.authorBetancor, Monica|0000-0003-1626-7458en_UK
local.rioxx.authorManchado, Manuel|en_UK
local.rioxx.authorTort, Lluis|en_UK
local.rioxx.authorTorrecillas, Silvia|en_UK
local.rioxx.authorZamorano, Maria J|en_UK
local.rioxx.authorIzquierdo, Marisol S|en_UK
local.rioxx.authorMontero, Daniel|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2018-06-24en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-06-23en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2018-06-24|en_UK
local.rioxx.filenameBenitez et al 2017.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0016-6480en_UK
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