Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25449
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dc.contributor.authorCarmona-Antoñanzas, Gretaen_UK
dc.contributor.authorSanti, Morenaen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.contributor.authorVera, LMen_UK
dc.date.accessioned2017-12-14T02:33:55Z-
dc.date.available2017-12-14T02:33:55Z-
dc.date.issued2017-12-31en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25449-
dc.description.abstractCircadian regulation of hepatic detoxification seems to be amongst the key roles of the biological clock. The liver is the major site for biotransformation, and in mammals, it contains several clock-controlled transcription factors such as PAR basic leucine zipper proteins (bZIP) and basic-helix-loop-helix (bHLH)-PAS family that act as circadian regulators of detoxification genes. This investigation explored the existence of daily and circadian expression of transcription factors involved in detoxification, as well as the temporal profile of a set of their target genes in zebrafish liver. In our study, zebrafish were able to synchronize to a light-dark (LD) cycle and displayed a diurnal pattern of activity. In addition, the expression of clock genes presented daily and circadian rhythmicity in liver. Apart from hlfa, the expression of PAR bZIP transcription factors also displayed daily rhythms, which appeared to be both light-dependent and clock-controlled, as circadian rhythms free-ran under constant conditions (continuous darkness, DD). Under LD, tefb, dbpa and dbpb expression peaked at the end of the darkness period whereas tefa showed peak levels of expression at the onset of the photophase. In addition, these four genes exhibited circadian expression under DD, with higher expression levels at the end of the subjective night. The expression of the bHLH-PAS transcription factor arh2 also showed circadian rhythmicity in zebrafish liver, peaking in the middle of the subjective night and approximately 3-4 hours before peak expression of the PAR bZIP genes. Regarding the detoxification genes, the major target gene of AhR, cyp1a, showed daily and circadian expression with an acrophase 2 hours after ahr2. Under LD, abcb4 also showed daily rhythmicity, with an acrophase 1-2 h after that of PAR bZIP factors during the transition between darkness and light phases, when zebrafish become active. However, the expression of six detoxification genes showed circadian rhythmicity under DD, including cyp1a and abcb4 as well as gstr1, mgst3a, abcg2 and sult2_st2. In all cases, the acrophases of these 3 genes were found during the second half of the subjective night, in phase with the PAR bZIP transcription factors. This suggested that their expression is clock-controlled, either directly by core clock genes or through transcription factors. This study presents new data demonstrating that the process of detoxification is under circadian control in fish. Results showed that time of day should be considered when designing toxicological studies or administering drugs to fish.en_UK
dc.language.isoenen_UK
dc.publisherTaylor and Francisen_UK
dc.relationCarmona-Antoñanzas G, Santi M, Migaud H & Vera L (2017) Light- and clock-control of genes involved in detoxification. Chronobiology International, 34 (8), pp. 1026-1041. https://doi.org/10.1080/07420528.2017.1336172en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Chronobiology International on 15 Jun 2017, available online: http://www.tandfonline.com/10.1080/07420528.2017.1336172.en_UK
dc.subjectZebrafish, liveren_UK
dc.subjectcircadianen_UK
dc.subjectPAR bZIPen_UK
dc.subjectbHLH-PASen_UK
dc.subjectdetoxification genesen_UK
dc.titleLight- and clock-control of genes involved in detoxificationen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-06-16en_UK
dc.rights.embargoreason[Light and clock control of detoxification.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1080/07420528.2017.1336172en_UK
dc.identifier.pmid28617195en_UK
dc.citation.jtitleChronobiology Internationalen_UK
dc.citation.issn1525-6073en_UK
dc.citation.issn0742-0528en_UK
dc.citation.volume34en_UK
dc.citation.issue8en_UK
dc.citation.spage1026en_UK
dc.citation.epage1041en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailluisa.veraandujar@stir.ac.uken_UK
dc.citation.date15/06/2017en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationMarche Polytechnic Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000415763500003en_UK
dc.identifier.scopusid2-s2.0-85020537152en_UK
dc.identifier.wtid527474en_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.contributor.orcid0000-0003-0999-055Xen_UK
dc.date.accepted2017-05-25en_UK
dcterms.dateAccepted2017-05-25en_UK
dc.date.filedepositdate2017-06-02en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorCarmona-Antoñanzas, Greta|en_UK
local.rioxx.authorSanti, Morena|en_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2018-06-16en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-06-15en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2018-06-16|en_UK
local.rioxx.filenameLight and clock control of detoxification.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1525-6073en_UK
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