Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25357
Appears in Collections:Aquaculture Journal Articles
Title: Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria
Authors: Pourahmad, Fazel
Richards, Randolph
Keywords: Sequence analyses
16S rRNA
rpoB
aquatic mycobacteria
Issue Date: 7-Sep-2016
Citation: Pourahmad F & Richards R (2016) Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria, Turkish Journal of Veterinary and Animal Sciences, 40 (4), pp. 406-416.
Abstract: The nucleotide sequences of partial 16S rRNA and bacterial RNA polymerase ß-subunit (rpoB) genes for 57 mycobacterial strains were determined. Compared to the 16S rRNA gene sequences, variable regions were scattered along the whole fragment sequence, indicating that therpoBgene is more polymorphic. Unlike 16S rRNA sequences, species variation was observed within M. fortuitum strains. The topology of therpoB-based phylogenetic tree was almost the same as that of the 16S rRNA sequence analysis. These results suggest that therpoBgene is a highly conserved gene, and taxonomical studies based on this gene may be comparable with similar studies based on the 16S rRNA gene. The overall mean distance forrpoB-gene?based sequences was 2.5 times greater than that of the 16S rRNA gene for all 57 mycobacterial strains examined. However, some slowly growing mycobacteria could not be differentiated based onrpoBgene sequences. Moreover, a bootstrap value above 70% was observed for 13 nodes, while this value was 14 nodes in the case of 16S rRNA sequences. To the best of our knowledge, this is the first investigation evaluating the use of 16S rRNA andrpoBsequence analyses for identification of aquatic mycobacteria obtained from diverse geographical locations.
DOI Link: http://dx.doi.org/10.3906/vet-1505-74
Rights: Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria, Fazel Pourahmad, Randolph Harvey Richards, Turkish Journal of Veterinary and Animal Sciences (2016) 40: 406-416, Copyright© Tubitak 2016

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