Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25192
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 Protein
Authors: Monroig, Oscar
de, Llanos Rosa
Varo, Inmaculada
Hontoria, Francisco
Tocher, Douglas R
Puig, Sergi
Navarro, Juan C
Keywords: biosynthesis
elongation of very long-chain fatty acids 4 protein
non-methylene- interrupted fatty acids
polyunsaturated fatty acids
Octopus vulgaris
stearoyl-CoA desaturase
Issue Date: 21-Mar-2017
Citation: Monroig O, de Llanos R, Varo I, Hontoria F, Tocher DR, Puig S & Navarro JC (2017) Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 Protein, Marine Drugs, 15 (3), Art. No.: 82.
Abstract: Polyunsaturated fatty acids (PUFAs) have been acknowledged as essential nutrients for cephalopods but the specific PUFAs that satisfy the physiological requirements are unknown. To expand our previous investigations on characterisation of desaturases and elongases involved in the biosynthesis of PUFAs and hence determine the dietary PUFA requirements in cephalopods, this study aimed to investigate the roles that a stearoyl-CoA desaturase (Scd) and an elongation of very long-chain fatty acid 4 (Elovl4) protein play in the biosynthesis of essential fatty acids (FAs). Our results confirmed the Octopus vulgaris Scd is a D9 desaturase with relatively high affinity towards saturated FAs with C18 chain lengths. Scd was unable to desaturate 20:1n-15 (D520:1) suggesting that its role in the biosynthesis of non-methylene interrupted FAs (NMI FAs) is limited to the introduction of the first unsaturation at D9 position. Interestingly, the previously characterised D5 fatty acyl desaturase was indeed able to convert 20:1n-9 (D1120:1) to D5,1120:2, an NMI FA previously detected in octopus nephridium. Additionally, Elovl4 was able to mediate the production of 24:5n-3 and thus can contribute to docosahexaenoic acid (DHA) biosynthesis through the Sprecher pathway. Moreover, the octopus Elovl4 was confirmed to play a key role in the biosynthesis of very long-chain (>C24) PUFAs.
DOI Link: http://dx.doi.org/10.3390/md15030082
Rights: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)

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