Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24982
Appears in Collections:Aquaculture Book Chapters and Sections
Title: Tissue Proteomics in Vascular Disease
Authors: Albalat, Amaya
Mullen, William
Husi, Holger
Mischak, Harald
Contact Email: amaya.albalat@stir.ac.uk
Editors: Touyz, RM
Schiffrin, EL
Citation: Albalat A, Mullen W, Husi H & Mischak H (2017) Tissue Proteomics in Vascular Disease. In: Touyz RM, Schiffrin EL (ed.). Hypertension: Tissue Proteomics in Vascular Disease. Methods in Molecular Biology, 1527, New York: Springer, pp. 53-60.
Keywords: Tissue proteomics
FASP method
MS/MS data analysis
Issue Date: 2017
Series/Report no.: Methods in Molecular Biology, 1527
Abstract: In vascular diseases, as in many other pathophysiological conditions, tissue proteins are subjected to a number of different changes such as protein expression, posttranslational modifications, and proteolytic cleavage. For this reason, the study of the tissue proteome is becoming an increasingly important tool in biological and clinical research. In this chapter, we describe in detail the methodology for the analysis of tryptic digested peptides from aortic tissue extracts from mice with the aim to elucidate differences in the proteome between control and case or vascular diseased tissue samples. The method encompasses the analysis of these complex extracts in a single chromatographic run allowing for increased reproducibility. We also outline the main workflow for the processing of the generated data by statistical and bioinformatic analysis.
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
URL: http://link.springer.com/protocol/10.1007/978-1-4939-6625-7_4
DOI Link: http://dx.doi.org/10.1007/978-1-4939-6625-7_4

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