|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom|
|Authors:||Ngo, Thao P H|
Thompson, Kim D
Verner-Jeffreys, David W
|Keywords:||Rainbow trout fry syndrome|
Bacterial cold water disease
|Citation:||Ngo TPH, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2017) Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom, Veterinary Microbiology, 201, pp. 216-224.|
|Abstract:||Flavobacterium psychrophilum is one of the most important bacterial pathogens affecting cultured rainbow trout (Oncorhynchus mykiss) and is increasingly causing problems in Atlantic salmon (Salmo salar L.) hatcheries. Little is known about the heterogeneity of F. psychrophilum isolates on UK salmonid farms. A total of 315 F. psychrophilum isolates, 293 of which were collected from 27 sites within the UK, were characterised using four genotyping methods and a serotyping scheme. A high strain diversity was identified among the isolates with 54 pulsotypes, ten (GTG)5-PCR types, two 16S rRNA allele lineages, seven plasmid profiles and three serotypes. Seven PFGE groups and 27 singletons were formed at a band similarity of 80%. PFGE group P (n=75) was found to be numerically predominant in eight sites within the UK. Two major PFGE clusters and 13 outliers were found at the band similarity of 40%. The predominant profile observed within the F. psychrophilum isolates examined was PFGE cluster II − (GTG)5-PCR type r1–16S rRNA lineage II − serotype Th (70/156 isolates examined, 45%). Co-existence of genetically and serologically heterogeneous isolates within each farm was detected, confounding the ability to control RTFS outbreaks. The occurrence over time (up to 11 years) of F. psychrophilum pulsotypes in three representative sites (Scot I, Scot III and Scot V) within Scotland was examined, potentially providing important epidemiological data for farm management and the development of site-specific vaccines.|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ngo TPH, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2017) Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom, Veterinary Microbiology, 201, pp. 216-224. DOI: 10.1016/j.vetmic.2017.01.032 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/|
|Manuscript (revised).pdf||331.2 kB||Adobe PDF||Under Embargo until 31/1/2018 Request a copy|
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