STORRE
STORRE: Stirling Online Research Repository
http://hdl.handle.net/1893/24948| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Suitability of DNA sampled from Nile tilapia skin mucus swabs as a template for ddRAD-based studies |
| Author(s): | Khanam, Taslima Taggart, John Wehner, Stefanie McAndrew, Brendan Penman, David |
| Contact Email: | d.j.penman@stir.ac.uk |
| Keywords: | Oreochromis niloticus Fish mucus DNA ddRADseq |
| Issue Date: | Mar-2017 |
| Date Deposited: | 8-Feb-2017 |
| Citation: | Khanam T, Taggart J, Wehner S, McAndrew B & Penman D (2017) Suitability of DNA sampled from Nile tilapia skin mucus swabs as a template for ddRAD-based studies. Conservation Genetics Resources, 9 (1), pp. 39-42. https://doi.org/10.1007/s12686-016-0614-z |
| Abstract: | For conservation, ethical and welfare reasons, minimally invasive DNA sampling is becoming increasingly important in animal genetics studies. Skin mucus swabs have been used as a source for fish DNA in PCR-based studies, but their suitability for high-throughput sequencing has not been examined. DNA was extracted from skin mucus, muscle and fin samples of two Nile tilapia and used in double-digest restriction-site associated DNA (ddRAD) sequencing. Approximately 16,000 and 9000 RAD loci were retrieved from de novo and reference-based analyses respectively. The numbers of RAD loci retrieved from three tissue sources were similar, with >83 % being shared among all three samples. Minor bacterial contamination was detected in a single muscle sample (0.07 % of the total RAD loci). The data indicates that DNA derived from skin mucus can be reliably used for ddRADseq and is likely to be applicable in other similar genomic analyses. |
| DOI Link: | 10.1007/s12686-016-0614-z |
| Rights: | The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. |
| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
| File | Description | Size | Format | |
|---|---|---|---|---|
| art3A10.10072Fs12686-016-0614-z.pdf | Fulltext - Published Version | 1.09 MB | Adobe PDF | Under Embargo until 2999-12-22 Request a copy |
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright |
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.