Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24948
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Suitability of DNA sampled from Nile tilapia skin mucus swabs as a template for ddRAD-based studies
Authors: Khanam, Taslima
Taggart, John
Wehner, Stefanie
McAndrew, Brendan
Penman, David
Contact Email: d.j.penman@stir.ac.uk
Keywords: Oreochromis niloticus
Fish mucus
DNA
ddRADseq
Issue Date: Mar-2017
Citation: Khanam T, Taggart J, Wehner S, McAndrew B & Penman D (2017) Suitability of DNA sampled from Nile tilapia skin mucus swabs as a template for ddRAD-based studies, Conservation Genetics Resources, 9 (1), pp. 39-42.
Abstract: For conservation, ethical and welfare reasons, minimally invasive DNA sampling is becoming increasingly important in animal genetics studies. Skin mucus swabs have been used as a source for fish DNA in PCR-based studies, but their suitability for high-throughput sequencing has not been examined. DNA was extracted from skin mucus, muscle and fin samples of two Nile tilapia and used in double-digest restriction-site associated DNA (ddRAD) sequencing. Approximately 16,000 and 9000 RAD loci were retrieved from de novo and reference-based analyses respectively. The numbers of RAD loci retrieved from three tissue sources were similar, with >83 % being shared among all three samples. Minor bacterial contamination was detected in a single muscle sample (0.07 % of the total RAD loci). The data indicates that DNA derived from skin mucus can be reliably used for ddRADseq and is likely to be applicable in other similar genomic analyses.
DOI Link: http://dx.doi.org/10.1007/s12686-016-0614-z
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