|Appears in Collections:||Aquaculture Journal Articles|
|Title:||Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay|
|Authors:||El, Wahed Ahmed Abd|
Sanabani, Sabri Saeed
Patriota, Joao Veras
Giorgi, Ricardo Rodrigues
Zanotto, Paolo M D A
Sall, Amadou A
|Citation:||El Wahed AA, Sanabani SS, Faye O, Pessoa R, Patriota JV, Giorgi RR, Patel P, Bohlken-Fascher S, Landt O, Niedrig M, Zanotto PMAD, Czerny C, Sall AA & Weidmann M (2017) Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay, PLOS Currents: Outbreaks (1).|
|Abstract:||Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-ofcare test is needed to detect the virus, especially at low resource settings. Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%. Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).|
|Rights:||This article is published under a Creative Commons Attribution License, enabling unrestricted distribution and use of the published materials, provided that its authors are properly credited.|
|Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay PLOS Currents Outbreaks.pdf||204.16 kB||Adobe PDF||View/Open|
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