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http://hdl.handle.net/1893/24855| Appears in Collections: | Aquaculture Journal Articles |
| Title: | Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay |
| Author(s): | El Wahed, Ahmed Abd Sanabani, Sabri Saeed Faye, Oumar Pessoa, Rodrigo Patriota, Joao Veras Giorgi, Ricardo Rodrigues Patel, Pranav Bohlken-Fascher, Susanne Landt, Olfert Niedrig, Matthias Zanotto, Paolo M D A Czerny, Claus-Peter Sall, Amadou A Weidmann, Manfred |
| Contact Email: | m.w.weidmann@stir.ac.uk |
| Issue Date: | 25-Jan-2017 |
| Date Deposited: | 27-Jan-2017 |
| Citation: | El Wahed AA, Sanabani SS, Faye O, Pessoa R, Patriota JV, Giorgi RR, Patel P, Bohlken-Fascher S, Landt O, Niedrig M, Zanotto PMDA, Czerny C, Sall AA & Weidmann M (2017) Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay. PLOS Currents: Outbreaks, (1), Art. No.: 9. https://doi.org/10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e |
| Abstract: | Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-ofcare test is needed to detect the virus, especially at low resource settings. Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%. Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering). |
| DOI Link: | 10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e |
| Rights: | This article is published under a Creative Commons Attribution License, enabling unrestricted distribution and use of the published materials, provided that its authors are properly credited. |
| Licence URL(s): | http://creativecommons.org/licenses/by/4.0/ |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay PLOS Currents Outbreaks.pdf | 204.16 kB | Adobe PDF | View/Open | |
| AbdElWahed-etal-PLOSCO-2017.pdf | Fulltext - Published Version | 204.16 kB | Adobe PDF | View/Open |
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