|Appears in Collections:||Aquaculture Journal Articles|
|Title:||Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay|
|Authors:||El, Wahed Ahmed Abd|
Sanabani, Sabri Saeed
Patriota, Joao Veras
Giorgi, Ricardo Rodrigues
Zanotto, Paolo M D A
Sall, Amadou A
|Citation:||El Wahed AA, Sanabani SS, Faye O, Pessoa R, Patriota JV, Giorgi RR, Patel P, Bohlken-Fascher S, Landt O, Niedrig M, Zanotto PMAD, Czerny C, Sall AA & Weidmann M (2017) Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay, PLOS Currents: Outbreaks (1).|
|Abstract:||Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-ofcare test is needed to detect the virus, especially at low resource settings. Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%. Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).|
|Rights:||This article is published under a Creative Commons Attribution License, enabling unrestricted distribution and use of the published materials, provided that its authors are properly credited.|
|Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay PLOS Currents Outbreaks.pdf||204.16 kB||Adobe PDF||View/Open|
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.