Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24809
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus
Authors: Shalaby, Mohamed A
El-Deeb, Ayman
El-Tholoth, Mohamed
Hoffmann, Donata
Czerny, Claus-Peter
Hufert, Frank T
Weidmann, Manfred
El, Wahed Ahmed Abd
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: Lumpy skin disease virus
Recombinase polymerase amplification assay
Point of need test
Cattle
Issue Date: 2-Nov-2016
Citation: Shalaby MA, El-Deeb A, El-Tholoth M, Hoffmann D, Czerny C, Hufert FT, Weidmann M & El Wahed AA (2016) Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus, BMC Veterinary Research, 12 (1), Art. No.: 244.
Abstract: Background  Lumpy skin disease virus (LSDV) is aCapripoxvirusinfecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed.  Results  The LSDV RPA assay was performed at 42 °C and detected down to 179 DNA copies/reaction in a maximum of 15 min. Unspecific amplification was observed with neither LSDV-negative samples (n= 12) nor nucleic acid preparations from orf virus, bovine papular stomatitis virus, cowpoxvirus, Peste des petits ruminants and Blue tongue virus (serotypes 1, 6 and 8). The clinical sensitivity of the LSDV RPA assay matched 100 % (n= 22) to real-time PCR results. In addition, the LSDV RPA assay detected sheep and goat poxviruses.  Conclusion  The LSDV RPA assay is a rapid and sensitive test that could be implemented in field or at quarantine stations for the identification of LSDV infected case.
DOI Link: http://dx.doi.org/10.1186/s12917-016-0875-5
Rights: © The Author(s). 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Files in This Item:
File Description SizeFormat 
Shalaby_etal_BMCVR_2017 (1).pdf576.49 kBAdobe PDFView/Open



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.