|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar)|
|Authors:||Bogevik, Andre S|
Tocher, Douglas R
Olsen, Rolf E
|Keywords:||bile salt-dependent lipase|
wax ester hydrolase
|Citation:||Bogevik AS, Tocher DR, Waagbo R & Olsen RE (2008) Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar), Aquaculture Nutrition, 14 (1), pp. 93-98.|
|Abstract:||Bile salt-dependent lipase (BSDL) is assumed to be the predominant lipid hydrolase in fish digestive tracts where it hydrolyses dietary triacylglycerols (TAG), sterol esters (SE) and wax esters (WE). BSDL is known to hydrolyse TAG at much faster rates than SE and WE in both fish and mammals. An assay for BSDL has previously been developed for rainbow trout (Oncorhynchus mykiss). However, this setup may not be valid in other fish species. Accordingly, the present study aimed at optimizing previous assays in rainbow trout for use on intestinal luminal contents of Atlantic salmon (Salmo salar L.). Crude intestinal extracts from midgut were desalted before the assay and concentrated bile salts supplemented. In general, the rank order for the degree of hydrolysis in Atlantic salmon was TAG > WE > SE. The optimal assay conditions were determined as being 100 lg protein, 125 lM lipid substrate and 20 mM bile salt (taurocholate) during the 4 h of incubation. Atlantic salmon and rainbow trout of 1500 g showed similar lipolytic activity, while salmon smolts of 300 g showed a significantly lower activity. Furthermore, the inhibition of intestinal lipase activities, especially triacylglycerol hydrolase and sterol ester hydrolase, observed in trout intestinal extracts at bile salt concentrations around 10 mM, was not observed in salmon. This could indicate that the activities in these two salmonids may display different enzyme biochemistry.|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.|
|Affiliation:||Matre Aquaculture Research Station|
University of Bergen
Matre Aquaculture Research Station
|Lipid hydrolases-technical note.pdf||340.49 kB||Adobe PDF||Under Embargo until 31/12/2999 Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact email@example.com providing details and we will remove the Work from public display in STORRE and investigate your claim.