Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2468
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A description of the origins, design and performance of the TRAITS-SGP Atlantic salmon Salmo salar L. cDNA microarray
Author(s): Taggart, John
Bron, James
Martin, Sam A M
Seear, Paul J
Hoyheim, Bjorn
Talbot, Richard T
Carmichael, Stephen N
Villeneuve, Laure
Sweeney, Glen E
Houlihan, Dominic F
Secombes, Christopher J
Tocher, Douglas R
Teale, Alan J
Contact Email: drt1@stir.ac.uk
Keywords: Atlantic salmon
DNA microarray
gene expression
lipid metabolism
immune response
smoltification
Issue Date: Jun-2008
Date Deposited: 14-Oct-2010
Citation: Taggart J, Bron J, Martin SAM, Seear PJ, Hoyheim B, Talbot RT, Carmichael SN, Villeneuve L, Sweeney GE, Houlihan DF, Secombes CJ, Tocher DR & Teale AJ (2008) A description of the origins, design and performance of the TRAITS-SGP Atlantic salmon Salmo salar L. cDNA microarray. Journal of Fish Biology, 72 (9), pp. 2071-2094. http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.2008.01876.x/abstract; https://doi.org/10.1111/j.1095-8649.2008.01876.x
Abstract: The origins, design, fabrication and performance of an Atlantic salmon microarray are described. The microarray comprises 16 950 Atlantic salmon-derived cDNA features, printed in duplicate and mostly sourced from pre-existing expressed sequence tag (EST) collections [SALGENE and salmon genome project (SGP)] but also supplemented with cDNAs from suppression subtractive hybridization libraries and candidate genes involved in immune response, protein catabolism, lipid metabolism and the parr–smolt transformation. A preliminary analysis of a dietary lipid experiment identified a number of genes known to be involved in lipid metabolism. Significant fold change differences (as low as 1.2x) were apparent from the microarray analysis and were confirmed by quantitative real-time polymerase chain reaction analysis. The study also highlighted the potential for obtaining artefactual expression patterns as a result of cross-hybridization of similar transcripts. Examination of the robustness and sensitivity of the experimental design employed demonstrated the greater importance of biological replication over technical (dye flip) replication for identification of a limited number of key genes in the studied system. The TRAITS (TRanscriptome Analysis of Important Traits of Salmon)–salmon genome project microarray has been proven, in a number of studies, to be a powerful tool for the study of key traits of Atlantic salmon biology. It is now available for use by researchers in the wider scientific community.
URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.2008.01876.x/abstract
DOI Link: 10.1111/j.1095-8649.2008.01876.x
Rights: Rights according to Exclusive Licence Form: http://www.blackwellpublishing.com/pdf/jfb_exclusive.pdf; Published in Journal of Fish Biology by Wiley-Blackwell / The Fisheries Society of the British Isles. Journal of Fish Biology, Volume 72, Issue 9, June 2008, pp. 2071 – 2094.; This article is the author's final, refereed version. The definitive version is available at www.blackwellsynergy.com

Files in This Item:
File Description SizeFormat 
taggart et al 2008.pdfFulltext - Accepted Version382.78 kBAdobe PDFView/Open
Taggart et al TRAITS paper.pdfFulltext - Accepted Version443.93 kBAdobe PDFView/Open



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.