Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24570
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Assessment of stress and nutritional biomarkers in cultured Octopus vulgaris paralarvae: Effects of geographical origin and dietary regime
Authors: Garrido, Diego
Varo, Inma
Morales, Amalia E
Hidalgo, M Carmen
Navarro, Juan Carlos
Hontoria, Francisco
Monroig, Oscar
Iglesias, Jose
Otero, Juan J
Estevez, Alicia
Perez, Josu
Martin, M Virginia
Rodriguez, Covadonga
Almansa, Eduardo
Cardenete, Gabriel
Contact Email: oscar.monroig@stir.ac.uk
Keywords: Antioxidant defences
Geographical origin
Heat shock protein
Marine phospholipids
Nucleic acid ratios
Octopus vulgaris paralarvae
Issue Date: 1-Feb-2017
Citation: Garrido D, Varo I, Morales AE, Hidalgo MC, Navarro JC, Hontoria F, Monroig O, Iglesias J, Otero JJ, Estevez A, Perez J, Martin MV, Rodriguez C, Almansa E & Cardenete G (2017) Assessment of stress and nutritional biomarkers in cultured Octopus vulgaris paralarvae: Effects of geographical origin and dietary regime, Aquaculture, 468 (Part 1), pp. 558-568.
Abstract: The common octopus (Octopus vulgaris) is a promising species for aquaculture diversification, but massive mortality during the first life-cycle stages (paralarvae) is the main bottleneck for its commercial production in captivity. The aim of this study was to assess stress and nutritional condition biomarkers (HSP70, ROS enzymes and lipid peroxidation) (RNA/DNA, RNA/protein, protein/DNA and protein) inO.vulgarisparalarvae from different geographical origins and fed withArtemiaenriched with marine phospholipids or microalgae (control group). To this end paralarvae were cultured for 30days, in three different centres in Spain (Tarragona-Mediterranean area, Tenerife-Central Atlantic area and Vigo-North Atlantic area), under the same protocol, and fed onArtemiaenriched with marine phospholipids (LC60) (Marine Lecithin LC 60®, PhosphoTech Laboratoires) or microalgae (control group). Dry weight and most biomarkers analysed in hatchlings showed significant differences related to their origin (centre). Fifteen day old paralarvae presented significant differences in specific growth rate (SGR) associated with their dietary regime, and also showed differences in biomarkers associated both with their geographical origin and dietary regime. The results suggest that the SGR of paralarvae were positively influenced by LC60, promoting growth and in agreement with the results of nutritional condition biomarkers (nucleic acids ratios). The antioxidant defences against oxidative damage were also boosted in the LC60 paralarvae group, possibly as a result of the elevated content in highly polyunsaturated fatty acids. In addition, the partial correlations found between biomarkers varied according to diet. However, no positive effect of LC60 on survival was observed. The high variability found among geographical origins, despite the use of the same rearing protocol, highlights the need to clarify the sources of such variability.
DOI Link: http://dx.doi.org/10.1016/j.aquaculture.2016.11.023
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Garrido D, Varo I, Morales AE, Hidalgo MC, Navarro JC, Hontoria F, Monroig O, Iglesias J, Otero JJ, Estevez A, Perez J, Martin MV, Rodriguez C, Almansa E & Cardenete G (2017) Assessment of stress and nutritional biomarkers in cultured Octopus vulgaris paralarvae: Effects of geographical origin and dietary regime, Aquaculture, 468 (Part 1), pp. 558-568. DOI: 10.1016/j.aquaculture.2016.11.023 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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