Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Mitogen-Activated Protein Kinase 14 Promotes AKI (Forthcoming/Available Online)
Authors: Ortiz, Alberto
Husi, Holger
Gonzalez-Lafuente, Laura
Valino-Rivas, Lara
Fresno, Manuel
Sanz, Ana Belen
Mullen, William
Albalat, Amaya
Mezzano, Sergio
Vlahou, Antonia
Mischak, Harald
Sanchez-Nino, Maria Dolores
Contact Email:
Keywords: acute kidney injury
non-canonical NFκB
tissue proteomics
Issue Date: 12-Sep-2016
Publisher: American Society of Nephrology
Citation: Ortiz A, Husi H, Gonzalez-Lafuente L, Valino-Rivas L, Fresno M, Sanz AB, Mullen W, Albalat A, Mezzano S, Vlahou A, Mischak H & Sanchez-Nino MD Mitogen-Activated Protein Kinase 14 Promotes AKI (Forthcoming/Available Online), Journal of the American Society of Nephrology.
Abstract: An improved understanding of pathogenic pathways may identify novel acute kidney injury (AKI) therapeutic approaches. Unbiased LC-MS/MS protein expression profiling combined with focused data mining identified MAP3K14 and non-canonical NFκB activation at the crossroads of the enriched pathways MAPK, ubiquitin-mediated proteolysis, chemokines, NFκB and apoptosis in the kidney cortex of experimental toxic AKI. In AKI the upstream kinase MAP3K14, the NFκB DNA binding heterodimer RelB/NFκB2, and proteins involved in NFκB2 p100 ubiquitination and proteasomal processing to p52, such as Ube2m and cullin1 were up-regulated. Immunohistochemistry localized MAP3K14 expression to tubular cells in experimental and human AKI. In vivo evidence of MAP3K14 activation in experimental folic acid-induced AKI consisted of NFκB2 p100 processing to p52, nuclear location and DNA binding of RelB and NFκB2. MAP3K14 activity-deficient aly/aly mice were protected from kidney dysfunction, inflammation and apoptosis in AKI induced by folic acid and from lethality in cisplatin-induced AKI. MAP3K14 siRNA targeting in cultured tubular cells decreased inflammation and cell death. Bone marrow transplantation experiments where consistent with a protective effect of renal cell MAP3K14 targeting. Cell culture and in vivo studies identified chemokines MCP-1, RANTES and CXCL10 as MAP3K14 targets in tubular cells, thus identifying potential mediators of the deleterious effect of MAP3K14 in kidney injury. In conclusion, MAP3K14 promotes kidney injury through promotion of inflammation and cell death and is a promising novel therapeutic target.
Type: Journal Article
DOI Link:
Rights: Publisher policy allows this work to be made available in this repository. Published in Journal of the American Society of Nephrology by American Society of Nephrology. The original publication is available at:
Affiliation: Universidad Autonoma de Madrid
University of Glasgow
Universidad Autonoma de Madrid
Universidad Autonoma de Madrid
Universidad Autonoma de Madrid
Universidad Autonoma de Madrid
University of Glasgow
Complex Systems
Universidad Austral de Chile
Biomedical Research Foundation Academy of Athens (BRFAA)
University of Glasgow
Universidad Autonoma de Madrid

Files in This Item:
File Description SizeFormat 
Ortiz AKI Proteomics R1 Tracked.pdf579.79 kBAdobe PDFView/Open
Ortiz A Suppl Fig R1.pdf823.58 kBAdobe PDFView/Open
Ortiz A Figures 1-8 R1.pdf7.95 MBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.