Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24342
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Real-Time RT-PCR Assays for Detection and Genotyping of West Nile Virus Lineages Circulating in Africa (Forthcoming)
Authors: Fall, Gamou
Faye, Martin
Weidmann, Manfred
Kaiser, Marco
Dupressoir, Anne
Ndiaye, El Hadj
Ba, Yamar
Diallo, Mawlouth
Faye, Ousmane
Diallo, Alpha Amadou
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: African lineages
genotyping
Real-time RT-PCR
West Nile virus
Issue Date: 2016
Publisher: Mary Ann Liebert
Citation: Fall G, Faye M, Weidmann M, Kaiser M, Dupressoir A, Ndiaye EH, Ba Y, Diallo M, Faye O & Diallo AA Real-Time RT-PCR Assays for Detection and Genotyping of West Nile Virus Lineages Circulating in Africa (Forthcoming), Vector Borne and Zoonotic Diseases.
Abstract: West Nile virus (WNV) is an emerging arbovirus, circulating worldwide between birds and mosquitoes, which impacts human and animal health. Since the mid-1990s, WNV outbreaks have emerged in Europe and America and represent currently the primary cause of encephalitis in the United States. WNV exhibits a great genetic diversity with at least eight different lineages circulating in the world, and four (1, 2, Koutango, and putative new) are present in Africa. These different WNV lineages are not readily differentiated by serology, and thus, rapid molecular tools are required for diagnostic. We developed here real-time RT-PCR assays for detection and genotyping of African WNV lineages. The specificity of the assays was tested using other flaviviruses circulating in Africa. The sensitivity was determined by testing serial 10-fold dilutions of viruses and RNA standards. The assays provided good specificity and sensitivity and the analytical detection limit was 10 copies/ reaction. The RT-PCR assays allowed the detection and genotyping of all WNV isolates in culture medium, human serum, and vertebrate tissues, as well as in field and experimental mosquito samples. Comparing the ratios of genome copy number/infectious virion (plaque-forming units), our study finally revealed new insight on the replication of these different WNV lineages in mosquito cells. Our RT-PCR assays are the first ones allowing the genotyping of all WNV African variants, and this may have important applications in surveillance and epidemiology in Africa and also for monitoring of their emergence in Europe and other continents.
Type: Journal Article
URI: http://hdl.handle.net/1893/24342
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Institut Pasteur de Dakar
Institut Pasteur de Dakar
Aquaculture
GenExpress GmbH
Institut Pasteur de Dakar
Institut Pasteur de Dakar
Institut Pasteur de Dakar
Institut Pasteur
Institut Pasteur de Dakar
Ministry of Health & Public Hygiene (Guinea)

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