Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2424
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene
Authors: Ocalewicz, Konrad
Mota-Velasco, Jose C
Campos-Ramos, Rafael
Penman, David
Contact Email: d.j.penman@stir.ac.uk
Keywords: cytogenetics
fish
fluorescence in-situ hybridization (FISH)
sex chromosomes
synaptonemal complex (SC)
Issue Date: Aug-2009
Publisher: Springer
Citation: Ocalewicz K, Mota-Velasco JC, Campos-Ramos R & Penman D (2009) FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene, Chromosome Research, 17 (6), pp. 773-782.
Abstract: Bivalent 1 of the synaptonemal complex (SC) in XY male Oreochromis niloticus shows an unpaired terminal region in early pachytene. This appears to be related to recombination suppression around a sex determination locus. To allow more detailed analysis of this, and unpaired regions in the karyotype of other Oreochromis species, we developed techniques for FISH on SC preparations, combined with DAPI staining. DAPI staining identified presumptive centromeres in SC bivalents, which appeared to correspond to the positions observed in the mitotic karyotype (the kinetochores could only be identified sporadically in silver stained EM SC images). Furthermore, two BAC clones containing Dmo (dmrt4) and OniY227 markers that hybridize to known positions in chromosome pair 1 in mitotic spreads (near the centromere, FLpter 0.25, and the putative sex determination locus, FLpter 0.57, respectively) were used as FISH probes on SCs to verify that the presumptive centromere identified by DAPI staining was located in the expected position. Visualization of both the centromere and FISH signals on bivalent 1 allowed the unpaired region to be positioned at Flpter 0.80 to 1.00, demonstrating that the unpaired region is located in the distal part of the long arm(s). Finally, differences between mitotic and meiotic measurements are discussed.
Type: Journal Article
URI: http://hdl.handle.net/1893/2424
DOI Link: http://dx.doi.org/10.1007/s10577-009-9071-9
Rights: Published in Chromosome Research by Springer. The original publication is available at www.springerlink.com
Affiliation: University of Stirling
University of Stirling
University of Stirling
Aquaculture

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