Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24207
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dc.contributor.authorLopes-Marques, Mónicaen_UK
dc.contributor.authorOzório, Rodrigoen_UK
dc.contributor.authorAmaral, Ricardoen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorCosta Castro, Luıs Filipeen_UK
dc.date.accessioned2017-05-31T23:35:20Z-
dc.date.available2017-05-31T23:35:20Z-
dc.date.issued2017-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24207-
dc.description.abstractThe Brazilian teleost Arapaima gigas is an iconic species of the Amazon. In recent years a significant effort has been put into the farming of arapaima to mitigate overfishing threats. However, little is known regarding the nutritional requirements of A. gigas in particular those for essential fatty acids including the long-chain polyunsaturated fatty acids (LC-PUFA) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The ability to biosynthesize LC-PUFA is dependent upon the gene repertoire of fatty acyl desaturases (Fads) and elongases (Elovl), as well as their fatty acid specificities. In the present study we characterized both molecularly and functionally an orthologue of the desaturase fatty acid desaturase 2 (fads2) from A. gigas. The isolated sequence displayed the typical desaturase features, a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. Functional characterization of A. gigas fads2 showed that, similar to other teleosts, the A. gigas fads2 exhibited a predominant Δ6 activity complemented with some capacity for Δ8 desaturation. Given that A. gigas belongs to one of the oldest teleostei lineages, the Osteoglossomorpha, these findings offer a significant insight into the evolution LC-PUFA biosynthesis in teleosts.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationLopes-Marques M, Ozório R, Amaral R, Tocher DR, Monroig O & Costa Castro LF (2017) Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 203, pp. 84-91. https://doi.org/10.1016/j.cbpb.2016.09.007en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Lopes-Marques M, Ozório R, Amaral R, Tocher DR, Monroig O & Costa Castro LF (2017) Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas, Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 203, pp. 84-91. DOI: 10.1016/j.cbpb.2016.09.007 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectArapaima gigasen_UK
dc.subjectFatty acid desaturase (Fads)en_UK
dc.subjectLong-chain polyunsaturated fatty acids (LC-PUFAs)en_UK
dc.subjectteleosts, evolutionen_UK
dc.titleMolecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigasen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2017-08-30en_UK
dc.rights.embargoreason[Lopes-Marques ARAPAIMA STORRE.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.cbpb.2016.09.007en_UK
dc.identifier.pmid27693628en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biologyen_UK
dc.citation.issn1096-4959en_UK
dc.citation.volume203en_UK
dc.citation.spage84en_UK
dc.citation.epage91en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailoscar.monroig@stir.ac.uken_UK
dc.citation.date29/09/2016en_UK
dc.contributor.affiliationUniversity of Portoen_UK
dc.contributor.affiliationUniversity of Portoen_UK
dc.contributor.affiliationFederal University of Goiasen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationUniversity of Portoen_UK
dc.identifier.isiWOS:000390626800010en_UK
dc.identifier.scopusid2-s2.0-84991737249en_UK
dc.identifier.wtid549923en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.date.accepted2016-09-27en_UK
dcterms.dateAccepted2016-09-27en_UK
dc.date.filedepositdate2016-09-14en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorLopes-Marques, Mónica|en_UK
local.rioxx.authorOzório, Rodrigo|en_UK
local.rioxx.authorAmaral, Ricardo|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorCosta Castro, Luıs Filipe|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-08-30en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2017-08-29en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2017-08-30|en_UK
local.rioxx.filenameLopes-Marques ARAPAIMA STORRE.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1096-4959en_UK
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