Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24106
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Detection of very long antisense transcripts by whole transcriptome RNA-Seq analysis of Listeria monocytogenes by semiconductor sequencing technology
Authors: Wehner, Stefanie
Mannala, Gopala K
Qing, Xiaoxing
Madhugiri, Ramakanth
Chakraborty, Trinad
Mraheil, Mobarak A
Hain, Torsten
Marz, Manja
Contact Email: stefanie.wehner@stir.ac.uk
Issue Date: 6-Oct-2014
Publisher: Public Library of Science
Citation: Wehner S, Mannala GK, Qing X, Madhugiri R, Chakraborty T, Mraheil MA, Hain T & Marz M (2014) Detection of very long antisense transcripts by whole transcriptome RNA-Seq analysis of Listeria monocytogenes by semiconductor sequencing technology, PLoS ONE, 9 (10), Art. No.: e108639.
Abstract: The Gram-positive bacterium Listeria monocytogenes is the causative agent of listeriosis, a severe food-borne infection characterised by abortion, septicaemia, or meningoencephalitis. L. monocytogenes causes outbreaks of febrile gastroenteritis and accounts for community-acquired bacterial meningitis in humans. Listeriosis has one of the highest mortality rates (up to 30%) of all food-borne infections. This human pathogenic bacterium is an important model organism for biomedical research to investigate cell-mediated immunity. L. monocytogenes is also one of the best characterised bacterial systems for the molecular analysis of intracellular parasitism. Recently several transcriptomic studies have also made the ubiquitous distributed bacterium as a model to understand mechanisms of gene regulation from the environment to the infected host on the level of mRNA and non-coding RNAs (ncRNAs). We have used semiconductor sequencing technology for RNA-seq to investigate the repertoire of listerial ncRNAs under extra- and intracellular growth conditions. Furthermore, we applied a new bioinformatic analysis pipeline for detection, comparative genomics and structural conservation to identify ncRNAs. With this work, in total, 741 ncRNA locations of potential ncRNA candidates are now known for L. monocytogenes, of which 611 ncRNA candidates were identified by RNA-seq. 441 transcribed ncRNAs have never been described before. Among these, we identified novel long non-coding antisense RNAs with a length of up to 5,400 nt e.g. opposite to genes coding for internalins, methylases or a high-affinity potassium uptake system, namely the kdpABC operon, which were confirmed by qRT-PCR analysis. RNA-seq, comparative genomics and structural conservation of L. monocytogenes ncRNAs illustrate that this human pathogen uses a large number and repertoire of ncRNA including novel long antisense RNAs, which could be important for intracellular survival within the infected eukaryotic host.
Type: Journal Article
URI: http://hdl.handle.net/1893/24106
DOI Link: http://dx.doi.org/10.1371/journal.pone.0108639
Rights: © 2014 Wehner et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Aquaculture
Giessen University
Giessen University
Giessen University
Giessen University
Giessen University
Giessen University
Philipps University of Marburg

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