Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/23996
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Cloning and characterization of Lxr and Srebp1, and their potential roles in regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus
Authors: Zhang, Qinghao
You, Cuihong
Liu, Fang
Zhu, Wendi
Wang, Shuqi
Xie, Dizhi
Monroig, Oscar
Tocher, Douglas R
Li, Yuanyou
Contact Email: d.r.tocher@stir.ac.uk
Keywords: Elovl
Fad
LC-PUFA biosynthesis
Lxr
Rabbitfish Siganus canaliculatus
Srebp1
Issue Date: Sep-2016
Publisher: Springer
Citation: Zhang Q, You C, Liu F, Zhu W, Wang S, Xie D, Monroig O, Tocher DR & Li Y (2016) Cloning and characterization of Lxr and Srebp1, and their potential roles in regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus, Lipids, 51 (9), pp. 1051-1063.
Abstract: Rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability to biosynthesize C20-22 long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, which is generally absent or low in marine teleosts. Thus, understanding the molecular basis of LC-PUFA biosynthesis in rabbitfish will contribute to efforts aimed at optimizing LC-PUFA biosynthesis in teleosts, especially marine species. In the present study, the importance of the transcription factors liver X receptor (Lxr) and sterol regulatory element-binding protein 1 (Srebp1) in regulation of LC-PUFA biosynthesis in rabbitfish was investigated. First, full-length cDNAs of Lxr and Srebp1 were cloned and characterized. The Lxr mRNA displayed a ubiquitous tissue expression pattern while Srebp1 was highly expressed in eyes, brain and intestine. In rabbitfish primary hepatocytes treated with Lxr agonist T0901317, the expression of Lxr and Srebp1 was activated, accompanied by elevated mRNA levels of Δ4 and Δ6/Δ5 fatty acyl desaturases (Fad), key enzymes of LC-PUFA biosynthesis, as well as peroxisome proliferator-activated receptor γ (Pparγ). In addition, Srebp1 displayed higher expression levels in liver of rabbitfish fed a vegetable oil diet or reared at 10 ppt salinity, which were conditions reported to increase the liver expression of Δ4 and Δ6/Δ5 Fad and LC-PUFA biosynthetic ability, than fish fed a fish oil diet or reared at 32 ppt, respectively. These results suggested that Lxr and Srebp1 are involved in regulation of LC-PUFA biosynthesis probably by promoting the expression of two Fads in rabbitfish liver, which, to our knowledge, is the first report in marine teleosts.
Type: Journal Article
URI: http://hdl.handle.net/1893/23996
DOI Link: http://dx.doi.org/10.1007/s11745-016-4176-3
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository; The final publication is available at Springer via http://dx.doi.org/10.1007/s11745-016-4176-3
Affiliation: Shantou University
Shantou University
Shantou University
Shantou University
Shantou University
Shantou University
Complex Systems
Aquaculture
Shantou University

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