Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/23741
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Eicosapentaenoic Acid, Arachidonic Acid and Eicosanoid Metabolism in Juvenile Barramundi Lates calcarifer
Authors: Salini, Michael J
Wade, Nicholas M
Araujo, Bruno C
Turchini, Giovanni M
Glencross, Brett
Contact Email: b.d.glencross@stir.ac.uk
Keywords: Arachidonic acid
Lipoxygenase
Cyclooxygenase
Eicosanoids
Gene expression
Molecular cloning
Issue Date: Aug-2016
Publisher: Springer
Citation: Salini MJ, Wade NM, Araujo BC, Turchini GM & Glencross B (2016) Eicosapentaenoic Acid, Arachidonic Acid and Eicosanoid Metabolism in Juvenile Barramundi Lates calcarifer, Lipids, 51 (8), pp. 973-988.
Abstract: A two part experiment was conducted to assess the response of barramundi (Lates calcarifer; initial weight=10.3±0.03g; mean±S.D.) fed one of five diets with varying eicosapentaenoic acid (diets 1, 5, 10, 15 and 20g/kg) or one of four diets with varying arachidonic acid (1, 6, 12, 18g/kg) against a fish oil control diet. After 6weeks of feeding, the addition of EPA or ARA did not impact on growth performance or feed utilisation. Analysis of the whole body fatty acids showed that these reflected those of the diets. The ARA retention demonstrated an inversely related curvilinear response to either EPA or ARA. The calculated marginal utilisation efficiencies of EPA and ARA were high (62.1 and 91.9% respectively) and a dietary ARA requirement was defined (0.012g/kg0.796/day). The partial cDNA sequences of genes regulating eicosanoid biosynthesis were identified in barramundi tissues, namely cyclooxygenase 1 (Lc COX1a, Lc COX1b), cyclooxygenase 2 (Lc COX2) and lipoxygenase (Lc ALOX-5). BothLc COX2andLc ALOX-5expression in the liver tissue were elevated in response to increasing dietary ARA, meanwhile expression levels ofLcCOX2and the mitochondrial fatty acid oxidation gene carnitine palmitoyltransferase 1 (LcCPT1a) were elevated in the kidney. A low level of EPA increased the expression ofLc COX1bin the liver. Consideration should be given to the EPA to ARA balance for juvenile barramundi in light of nutritionally inducible nature of the cyclooxygenase and lipoxygenase enzymes.
Type: Journal Article
URI: http://hdl.handle.net/1893/23741
DOI Link: http://dx.doi.org/10.1007/s11745-016-4167-4
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository; The final publication is available at Springer via http://dx.doi.org/10.1007/s11745-016-4167-4
Affiliation: Deakin University
CSIRO Marine and Atmospheric Research
University of Sao Paulo
Deakin University
Aquaculture

Files in This Item:
File Description SizeFormat 
Defining the quantitative ARA and EPA_FINAL.pdf904.65 kBAdobe PDFUnder Embargo until 1/9/2017     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.