Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/23540
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings
Authors: Reis, Diana B
Acosta, Nieves Guadalupe
Almansa, Eduardo
Tocher, Douglas R
Andrade, Jose Pedro
Sykes, Antonio V
Rodriguez, Covadonga
Contact Email: drt1@stir.ac.uk
Keywords: Hatchlings
Metabolism
Octopus vulgaris
Phospholipids
Sepia officinalis
Issue Date: Oct-2016
Publisher: Elsevier
Citation: Reis DB, Acosta NG, Almansa E, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2016) Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings, Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 200, pp. 62-68.
Abstract: The objective of the present study was to characterise the fatty acid (FA) profiles of the major phospholipids, of Octopus vulgaris and Sepia officinalis hatchlings, namely phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine (PE); and to evaluate the capability of both cephalopod species on dietary phospholipid remodelling. Thus, O. vulgaris and S. officinalis hatchlings were in vivo incubated with 0.3μM of L-∝-1-palmitoyl-2-[1-14C]arachidonyl-PC or L-∝-1-palmitoyl-2-[1-14C]arachidonyl-PE. Octopus and cuttlefish hatchlings phospholipids showed a characteristic FA profiles with PC presenting high contents of 16:0 and 22:6n-3 (DHA); PS having high 18:0, DHA and 20:5n-3 (EPA); PI a high content of saturated FA; and PE showing high contents of DHA and EPA. Interestingly, the highest content of 20:4n-6 (ARA) was found in PE rather than PI. Irrespective of the phospholipid in which [1-14C]ARA was initially bound (either PC or PE), the esterification pattern of [1-14C]ARA in octopus lipids was similar to that found in their tissues with high esterification of this FA into PE. In contrast, in cuttlefish hatchlings [1-14C]ARA was mainly recovered in the same phospholipid that was provided. These results showed a characteristic FA profiles in the major phospholipids of the two species, as well as a contrasting capability to remodel dietary phospholipids, which may suggest a difference in phospholipase activities.
Type: Journal Article
URI: http://hdl.handle.net/1893/23540
DOI Link: http://dx.doi.org/10.1016/j.cbpb.2016.06.001
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Reis DB, Acosta NG, Almansa E, Tocher DR, Andrade JP, Sykes AV & Rodriguez C (2016) Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings, Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 200, pp. 62-68. DOI: 10.1016/j.cbpb.2016.06.001 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Affiliation: University of Algarve
University of La Laguna
Spanish Institute of Oceanography (IEO)
Aquaculture
University of Algarve
University of Algarve
University of La Laguna

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