Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Isolation and functional characterisation of a fads2 in rainbow trout (Oncorhynchus mykiss) with ∆5 desaturase activity
Authors: Abdul, Hamid Noor Khalidah
Carmona-Antonanzas, Greta
Monroig, Oscar
Tocher, Douglas R
Turchini, Giovanni M
Donald, John
Contact Email:
Issue Date: 4-Mar-2016
Publisher: Public Library of Science
Citation: Abdul Hamid NK, Carmona-Antonanzas G, Monroig O, Tocher DR, Turchini GM & Donald J (2016) Isolation and functional characterisation of a fads2 in rainbow trout (Oncorhynchus mykiss) with ∆5 desaturase activity, PLoS ONE, 11 (3), Art. No.: e0150770.
Abstract: Rainbow trout, Oncorhynchus mykiss, are intensively cultured globally. Understanding their requirement for long-chain polyunsaturated fatty acids (LC-PUFA) and the biochemistry of the enzymes and biosynthetic pathways required for fatty acid synthesis is important and highly relevant in current aquaculture. Most gnathostome vertebrates have two fatty acid desaturase (fads) genes with known functions in LC-PUFA biosynthesis and termed fads1 and fads2. However, teleost fish have exclusively fads2 genes. In rainbow trout, a fads2 cDNA had been previously cloned and found to encode an enzyme with Δ6 desaturase activity. In the present study, a second fads2 cDNA was cloned from the liver of rainbow trout and termed fads2b. The full-length mRNA contained 1578 nucleotides with an open reading frame of 1365 nucleotides that encoded a 454 amino acid protein with a predicted molecular weight of 52.48 kDa. The predicted Fads2b protein had the characteristic traits of the microsomal Fads family, including an N-terminal cytochrome b5 domain containing the heme-binding motif (HPPG), histidine boxes (HDXGH, HFQHH and QIEHH) and three transmembrane regions. The fads2b was expressed predominantly in the brain, liver, intes- tine and pyloric caeca. Expression of the fads2b in yeast generated a protein that was found to specifically convert eicosatetraenoic acid (20:4n-3) to eicosapentaenoic acid (20:5n-3), and therefore functioned as a Δ5 desaturase. Therefore, rainbow trout have two fads2 genes that encode proteins with Δ5 and Δ6 desaturase activities, respectively, which enable this species to perform all the desaturation steps required for the biosynthesis of LC- PUFA from C18 precursors.
Type: Journal Article
DOI Link:
Rights: © 2016 Abdul Hamid et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Deakin University
Complex Systems
Deakin University
Deakin University

Files in This Item:
File Description SizeFormat 
Abdul Hamid 2016 Trout Delta5.pdf4.02 MBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.