Effects of on-demand feeding on sea urchin larvae (Paracentrotus lividus; Lamarck, 1816), development, survival and microalgae utilization

Abstract

This study compared the growth of sea urchinParacentrotus lividuslarvae cultured using two different rearing methods: a variable method based on a variable amount of feed (microalgae) and seawater exchange (30% or 50%) established according to the phytoplankton concentration in the larval cultures and a fixed method characterized by a fixed amount of feed and seawater exchange. Three microalgae diets,Isochrysissp. (Tahitian strain, T-Iso),Chaetoceros gracilisand a 50:50 mixed diet, were tested with both rearing methods. Larval development and survival were assessed at the 6-arm pluteus stage (P6), competence (Cp) and metamorphosis (Mt). Data showed that the variable method reduced the requirements for phytoplankton and seawater exchange. Indeed, through the optimization of feed rations, it was possible to reduce the production of debris and settled phytoplankton, minimizing the need for water exchanges. Higher larval survival resulted at Cp and Mt stages for those reared with the variable method as opposed to the fixed one. Survival and development were also influenced by the tested dietary treatments: at Mt stage, the mixed diet resulted in a higher larval survival (63.3±8.9%) than T-Iso (19.7±12.1%) andC. gracilis(23.4±15.1%) (P<0.05). These results suggest that the use of the variable method improves the larval survival and development and also it reduces resource consumption (phytoplankton, seawater use and work effort), which in turn could potentially improve the hatchery production ofP.lividus.