|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs|
Tocher, Douglas R
essential fatty acids
long-chain polyunsaturated fatty acids
|Citation:||Oboh A, Betancor M, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs, Aquaculture, 462, pp. 70-79.|
|Abstract:||Fish differ in their capacity for endogenous synthesis of long-chain (C20-24) polyunsaturated fatty acids (LC-PUFA) from dietary C18 precursors (α-linolenic and linoleic acids). Understanding this capacity is of benefit to fish feed formulation. This, together with the importance of fish as the primary source of omega-3 LC-PUFA in the human diet has necessitated the rigorous study of the biochemical and molecular mechanisms involved in the LC-PUFA biosynthesis pathway in fish species. Studies have shown the potential of a species for LC-PUFA biosynthesis is associated with the complement and function of fatty acyl desaturase (fads) and elongase of very long chain fatty acid (elovl) gene it possesses. The present study therefore aimed to investigate these genes in the African catfish (Clarias gariepinus), the most commercially important farmed fish species in Sub-Saharan Africa. A fads2 and an elovl2 cDNA were cloned containing open reading frames (ORF) of 1338 base pair (bp) and 864 bp specifying proteins of 445 and 287 amino acids, respectively. Functional characterisation by heterologous expression in yeast showed that the Fads2 was bifunctional with ∆5∆6 activities catalysing the desaturation of both 18:3n-3 and 20:4n-3 and their corresponding n-6 fatty acids, 18:2n-6 and 20:3n-6. The Elovl2 showed activity towards C18, C20 and C22 PUFA with highest activity towards C20 and C22 PUFA. Tissue expression analysis showed a typical freshwater species expression pattern; higher expression in the liver compared to brain and all other tissues with the exception of elovl5 which showed highest expression in the intestine. Consistent with feeding studies of typical freshwater fish species that show their essential fatty acid requirement can be satisfied by dietary C18 PUFA, the present study confirms that the LC-PUFA biosynthesis pathway is active in the African catfish C. gariepinus.|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Oboh A, Betancor M, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs, Aquaculture, 462, pp. 70-79. DOI: 10.1016/j.aquaculture.2016.05.018 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/|
|Oboh et al Aquaculture 2016 STORRE.pdf||2.02 MB||Adobe PDF||Under Embargo until 12/5/2017 Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.