Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/23159
Full metadata record
DC FieldValueLanguage
dc.contributor.authorVera, LMen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.date.accessioned2016-08-20T01:19:47Z-
dc.date.available2016-08-20T01:19:47Z-
dc.date.issued2016en_UK
dc.identifier.urihttp://hdl.handle.net/1893/23159-
dc.description.abstractDaily variation in the absorption, metabolism and excretion of toxic substances will ultimately determine the actual concentration to which the cells and tissues are exposed. In aquaculture, Atlantic salmon (Salmo salar) can be frequently exposed to hydrogen peroxide (H2O2) to treat topical skin and gill infections, particularly in relation to parasitic infections (e.g. sea liceLepeophtheirus salmonisand amoebic gill disease caused byNeoparamoeba perurans). It is well accepted that the time of administration influences pharmacodynamics and pharmacokinetics of drugs which in turn affects their efficacy and toxicity. Consequently, a better understanding of drug side effects as a function of time of day exposure would help to improve treatment efficacy and fish welfare. To this end, salmon were exposed to H2O2(1500 mg/L) for 20 min at six different times of the day during a 24-h cycle and we investigated the time-dependent effects of exposure on physiological stress (glucose, lactate and cortisol) and antioxidant enzyme expression (gpx1, cat, Mn-sodandhsp70) in liver and gills. In addition, at each sampling point, 8 control fish were also sampled. Our results revealed that the time of administration of H2O2caused significant differences in the induction of both physiological and oxidative stress responses. Glucose and lactate were higher in the treated fish during daytime whereas cortisol levels appeared to be systematically increased (>1000 ng/mL) after H2O2treatment irrespective of exposure time, although differences with control levels were higher during the day. In liver, gene expression of antioxidant enzymes displayed daily rhythmicity in both treated and control groups and showed higher mRNA expression levels in salmon treated with H2O2at ZT6 (6 h after lights onset). In gills, rhythmic expression was only found forgpx1in the control fish and forhsp70andMn-sodin the treated groups. However, in the treated salmon, higher gene expression levels of all the investigated enzymes were also observed at ZT6-10. Clock gene expression showed rhythmicity only in the liver in accordance with the daily rhythm of enzyme expression observed in this tissue. Altogether, this study provides first evidence of chronotoxicity in Atlantic salmon treated with H2O2and suggests increased sublethal toxic effect during the first half of the day. These results have direct relevance to the salmon and broader aquaculture industry by optimising the timing of treatment administration, opening the door to chronotherapy to treat fish diseases.en_UK
dc.language.isoenen_UK
dc.publisherTaylor and Francisen_UK
dc.relationVera L & Migaud H (2016) Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner. Chronobiology International, 33 (5), pp. 530-542. https://doi.org/10.3109/07420528.2015.1131164en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Chronobiology International on 08 April 2016, available online: http://www.tandfonline.com/10.3109/07420528.2015.1131164en_UK
dc.subjectAtlantic salmonen_UK
dc.subjectchronotherapyen_UK
dc.subjectchronotoxicityen_UK
dc.subjectdaily rhythmsen_UK
dc.subjecthydrogen peroxideen_UK
dc.subjectoxidative stressen_UK
dc.subjectstress responseen_UK
dc.titleHydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manneren_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2017-04-09en_UK
dc.rights.embargoreason[Hydrogen_peroxide_chronotoxicity_in_salmon.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.3109/07420528.2015.1131164en_UK
dc.identifier.pmid27058450en_UK
dc.citation.jtitleChronobiology Internationalen_UK
dc.citation.issn1525-6073en_UK
dc.citation.issn0742-0528en_UK
dc.citation.volume33en_UK
dc.citation.issue5en_UK
dc.citation.spage530en_UK
dc.citation.epage542en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailherve.migaud@stir.ac.uken_UK
dc.citation.date08/04/2016en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000379779000006en_UK
dc.identifier.scopusid2-s2.0-84964055112en_UK
dc.identifier.wtid572604en_UK
dc.contributor.orcid0000-0003-0999-055Xen_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.date.accepted2015-12-08en_UK
dcterms.dateAccepted2015-12-08en_UK
dc.date.filedepositdate2016-04-26en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-04-09en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2017-04-08en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2017-04-09|en_UK
local.rioxx.filenameHydrogen_peroxide_chronotoxicity_in_salmon.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0742-0528en_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Hydrogen_peroxide_chronotoxicity_in_salmon.pdfFulltext - Accepted Version841.99 kBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.