Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22888
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Evolutionary functional elaboration of the Elovl2/5 gene family in chordates
Authors: Monroig, Oscar
Lopes-Marques, Mónica
Navarro, Juan Carlos
Hontoria, Francisco
Ruvio, Raquel
Santos, Miguel
Venkatesh, Byrappa
Tocher, Douglas R
Costa, Castro Luıs Filipe
Contact Email: oscar.monroig@stir.ac.uk
Keywords: Chordates
Long-chain polyunsaturated fatty acids
Evolution
Elongase
Issue Date: 9-Feb-2016
Publisher: Nature Publishing Group
Citation: Monroig O, Lopes-Marques M, Navarro JC, Hontoria F, Ruvio R, Santos M, Venkatesh B, Tocher DR & Costa Castro LF (2016) Evolutionary functional elaboration of the Elovl2/5 gene family in chordates, Scientific Reports, 6, Art. No.: 20510.
Abstract: The biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA) provides an intriguing example on how multi-enzymatic cascades evolve. Essential LC-PUFA, such as arachidonic, eicosapentaenoic, and docosahexaenoic acids (DHA), can be acquired from the diet but are also endogenously retailored from C18 precursors through consecutive elongations and desaturations catalyzed, respectively, by fatty acyl elongase and desaturase enzymes. The molecular wiring of this enzymatic pathway de nes the ability of a species to biosynthesize LC-PUFA. Exactly when and how in animal evolution a functional LC-PUFA pathway emerged is still elusive. Here we examine key components of the LC-PUFA cascade, the Elovl2/Elovl5 elongases, from amphioxus, an invertebrate chordate, the sea lamprey, a representative of agnathans, and the elephant shark, a basal jawed vertebrate. We show that Elovl2 and Elovl5 emerged from genome duplications in vertebrate ancestry. The single Elovl2/5 from amphioxus e ciently elongates C18 and C20 and, to a marked lesser extent, C22 LC-PUFA. Lamprey is incapable of elongating C22 substrates. The elephant shark Elovl2 showed that the ability to e ciently elongate C22 PUFA and thus to synthesize DHA through the Sprecher pathway, emerged in the jawed vertebrate ancestor. Our ndings illustrate how non-integrated “metabolic islands” evolve into fully wired pathways upon duplication and neofunctionalization.
Type: Journal Article
URI: http://hdl.handle.net/1893/22888
DOI Link: http://dx.doi.org/10.1038/srep20510
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Affiliation: Complex Systems
University of Porto
Institute of Aquaculture Torre de la Sal
Institute of Aquaculture Torre de la Sal
University of Porto
University of Porto
Institute of Molecular and Cell Biology - A*STAR
Aquaculture
University of Porto

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